Vps9d1-KO 基因敲除小鼠

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产品名称

Vps9d1-KO 基因敲除小鼠

产品编号

S-KO-13911

品系全称

C57BL/6JCya-Vps9d1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-72325-Vps9d1-B6J-VA

品系状态

使用本品系发表的文献需注明: Vps9d1-KO 基因敲除小鼠 mice (Strain S-KO-13911) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
VPS9 domain containing 1
基因别称
1300018I17Rik,2410004N05Rik,5DOS1,Vsp9d1
染色体号
Chr 8 (Mouse)
转录本 ID
NCBI: NM_001310661.1 | Ensembl: ENSMUST00000118279
修饰方式
全身性基因敲除
靶向范围
Exon 3~15
敲除长度
~7919 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
Vps9d1(VPS9 domain-containing protein 1)是小鼠中编码一个含有VPS9结构域的蛋白质,该结构域常见于鸟苷酸交换因子(GEF),可激活特定Rab GTP酶,参与调控内吞和膜运输过程。Vps9d1 基因位于小鼠8号染色体8E1区域,由15个外显子组成,编码区长度约为1950 bp,编码649个氨基酸的蛋白质,其功能被认为与早期内体形成和膜运输密切相关 。

研究表明,Vps9d1 可特异性激活Rab22A GTP酶,进而调控管状内体的形成,是维持细胞内运输网络完整性的重要因子之一 。此外,Vps9d1 在多种组织中均有表达,尤其在脑组织中表达较高,提示其可能在神经系统中具有特定功能 。

综上,Vps9d1 是一个参与膜运输调控的关键基因,具有潜在的神经生物学功能,是研究内吞作用和相关疾病机制的重要候选基因。

参考文献:
1. Gao, Xueren, Zhang, Shulong, Wang, Xiaoting. 2022. VPS9D1-AS1 gene rs7206570 polymorphism associated with the clinical stage of colorectal cancer and binding with hsa-miR-361-3p. In Human cell, 35, 522-527. doi:10.1007/s13577-021-00658-1. https://pubmed.ncbi.nlm.nih.gov/35022999/
2. Yang, Lei, Dong, Xichen, Liu, Zheng, Qu, Hao, Wang, Zhenjun. 2022. VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8+ T cell killing in colorectal cancer. In eLife, 11, . doi:10.7554/eLife.79811. https://pubmed.ncbi.nlm.nih.gov/36458816/
3. Chen, Lu, Shen, Meng. 2022. LncRNA VPS9D1-AS1 Sponging miR-520a-5p Contributes to the Development of Uterine Corpus Endometrial Carcinoma by Enhancing BIRC5 Expression. In Molecular biotechnology, 64, 1328-1339. doi:10.1007/s12033-022-00510-3. https://pubmed.ncbi.nlm.nih.gov/35619019/
4. Liu, Wei. 2021. Long non-coding RNA VPS9D1-AS1 promotes growth of colon adenocarcinoma by sponging miR-1301-3p and CLDN1. In Human cell, 34, 1775-1787. doi:10.1007/s13577-021-00604-1. https://pubmed.ncbi.nlm.nih.gov/34519940/
5. Zhou, Ning, Li, Sheng, Wu, Deming, Tang, Futian, Li, Yumin. 2021. The lncRNA VPS9D1-AS1 Promotes Hepatocellular Carcinoma Cell Cycle Progression by Regulating the HuR/CDK4 Axis. In DNA and cell biology, 40, 1278-1289. doi:10.1089/dna.2021.0235. https://pubmed.ncbi.nlm.nih.gov/34558987/
6. Peng, Tian-Fang, Zhou, Yan-Jie, Zhou, Jian, Li, Xin-Chun, Ouyang, Qiang. 2022. Long non-coding RNA VPS9D1-AS1 enhances proliferation, invasion, and epithelial-mesenchymal transition in endometrial cancer via miR-377-3p/SGK1. In The Kaohsiung journal of medical sciences, 38, 1048-1059. doi:10.1002/kjm2.12606. https://pubmed.ncbi.nlm.nih.gov/36245426/
7. Chen, Min, Wu, Xiaoliang, Ma, Wenjuan, Wang, Jiahong, Yang, Xianzi. . Decreased expression of lncRNA VPS9D1-AS1 in gastric cancer and its clinical significance. In Cancer biomarkers : section A of Disease markers, 21, 23-28. doi:10.3233/CBM-170172. https://pubmed.ncbi.nlm.nih.gov/29036784/
8. Ren, Wei, Ouyang, Ling. 2024. Long noncoding RNA VPS9D1-AS1 promotes the progression of endometrial cancer via regulation of the miR-187-3p/S100A4 axis. In Environmental toxicology, 39, 4447-4458. doi:10.1002/tox.24351. https://pubmed.ncbi.nlm.nih.gov/38953363/
9. Wu, Chenguang, Chen, Jian, Wang, Dong. . LncRNA VPS9D1-AS1 regulates miR-187-3p/fibroblast growth factor receptor-like 1 axis to promote proliferation, migration, and invasion of prostate cancer cells. In The Chinese journal of physiology, 66, 295-305. doi:10.4103/cjop.CJOP-D-23-00054. https://pubmed.ncbi.nlm.nih.gov/37929340/
10. Xiao, Shishan, Xu, Na, Ding, Qian, Zha, Yan, Zhu, Hongqian. . LncRNA VPS9D1-AS1 promotes cell proliferation in acute lymphoblastic leukemia through modulating GPX1 expression by miR-491-5p and miR-214-3p evasion. In Bioscience reports, 40, . doi:10.1042/BSR20193461. https://pubmed.ncbi.nlm.nih.gov/32808668/