Riox1-KO 基因敲除小鼠

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产品名称

Riox1-KO 基因敲除小鼠

产品编号

S-KO-13760

品系全称

C57BL/6JCya-Riox1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-71952-Riox1-B6J-VA

品系状态

使用本品系发表的文献需注明: Riox1-KO 基因敲除小鼠 mice (Strain S-KO-13760) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
ribosomal oxygenase 1
基因别称
2410016O06Rik,MAPJD,NO66
染色体号
Chr 12 (Mouse)
转录本 ID
NCBI: NM_023633 | Ensembl: ENSMUST00000053744
修饰方式
全身性基因敲除
靶向范围
Exon 1
敲除长度
~1.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1919202Mice homozygous for a knock-out allele activated in mesenchyme exhibit increased body length and weight, increased ossification with increased bone mass, bone mineral density, and volume, increased osteoblasts; and decrease osteoclasts.
Riox1,也称为NO66,是一种重要的核仁蛋白,属于JmjC结构域蛋白家族,具有组蛋白去甲基化酶和核糖体蛋白Rpl8羟化酶活性。Riox1在多种生物学过程中发挥作用,包括基因转录、RNA加工和翻译等。Riox1在核仁中定位,参与核糖体生物合成和成熟,以及组蛋白修饰的调控。

研究表明,Riox1在多种疾病中发挥重要作用。在骨质疏松症中,Riox1在成骨细胞中的过度表达导致成年小鼠的骨质疏松症,包括骨量、骨量密度和骨矿物质密度的降低,以及骨髓中脂肪滴的积累。Riox1抑制成骨细胞分化和骨形成,导致骨质疏松症的发生[2]。此外,Riox1在成肌细胞中的过度表达导致慢性肾病(CKD)小鼠肌肉质量的丧失和蛋白质合成的抑制。Riox1通过其去甲基化酶活性抑制核糖体生物合成,导致肌肉蛋白质合成的减少。抑制Riox1的表达可以阻断CKD引起的肌肉质量丧失和改善蛋白质合成[6]。

Riox1在癌症中也发挥重要作用。在食管鳞状细胞癌(ESCC)中,Riox1的表达与患者预后相关。高水平的Riox1表达与较差的总生存期(OS)相关,提示Riox1可能是ESCC的预后标志物。此外,Riox1在胶质瘤和胶质母细胞瘤组织中的高水平表达也与患者预后不良相关。Riox1通过调节表皮生长因子受体(EGFR)的表达和细胞增殖,在胶质瘤细胞中发挥促癌作用[3,7]。

Riox1在急性髓性白血病(AML)中也发挥重要作用。在AML细胞系中,Riox1的表达与细胞的成熟和增殖相关。Riox1的表达水平与细胞分化状态相关,高水平的Riox1表达与细胞成熟相关,而低水平的Riox1表达与细胞增殖相关。Riox1通过调节基因表达,影响细胞的增殖和成熟,从而影响AML的发生发展[8]。

综上所述,Riox1是一种重要的核仁蛋白,参与核糖体生物合成和成熟,以及组蛋白修饰的调控。Riox1在多种疾病中发挥重要作用,包括骨质疏松症、CKD、ESCC和AML。Riox1的研究有助于深入理解核仁蛋白的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略[1,4,5,9,10]。

参考文献:
1. Bräuer, Katharina E, Brockers, Kevin, Moneer, Jasmin, Lengeling, Andreas, Wolf, Alexander. 2018. Phylogenetic and genomic analyses of the ribosomal oxygenases Riox1 (No66) and Riox2 (Mina53) provide new insights into their evolution. In BMC evolutionary biology, 18, 96. doi:10.1186/s12862-018-1215-0. https://pubmed.ncbi.nlm.nih.gov/29914368/
2. Chen, Qin, Sinha, Krishna M, de Crombrugghe, Benoit, Krahe, Ralf. 2023. Osteoblast-Specific Overexpression of Nucleolar Protein NO66/RIOX1 in Mouse Embryos Leads to Osteoporosis in Adult Mice. In Journal of osteoporosis, 2023, 8998556. doi:10.1155/2023/8998556. https://pubmed.ncbi.nlm.nih.gov/36660551/
3. Zhao, Mengnan, Jin, Xing, Chen, Zhencong, Wang, Hao, Wang, Qun. . Weighted Correlation Network Analysis of Cancer Stem Cell-Related Prognostic Biomarkers in Esophageal Squamous Cell Carcinoma. In Technology in cancer research & treatment, 21, 15330338221117003. doi:10.1177/15330338221117003. https://pubmed.ncbi.nlm.nih.gov/35899307/
4. Häfliger, Irene M, Spengeler, Mirjam, Seefried, Franz R, Drögemüller, Cord. 2022. Four novel candidate causal variants for deficient homozygous haplotypes in Holstein cattle. In Scientific reports, 12, 5435. doi:10.1038/s41598-022-09403-6. https://pubmed.ncbi.nlm.nih.gov/35361830/
5. Qin, Zuliang, Li, Zhiqiang, Yang, Shuangyi, Wang, Deshou, Sun, Lina. 2021. Genome-wide identification, evolution of histone lysine demethylases (KDM) genes and their expression during gonadal development in Nile tilapia. In Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 257, 110674. doi:10.1016/j.cbpb.2021.110674. https://pubmed.ncbi.nlm.nih.gov/34624518/
6. Zhang, Liping, Chen, Qin, Chen, Zihong, Garibotto, Giacomo, Mitch, William E. 2020. Mechanisms Regulating Muscle Protein Synthesis in CKD. In Journal of the American Society of Nephrology : JASN, 31, 2573-2587. doi:10.1681/ASN.2019121277. https://pubmed.ncbi.nlm.nih.gov/32764136/
7. Wang, Qing, Liu, Pei Y, Liu, Tao, Lan, Qing. . The Histone Demethylase NO66 Induces Glioma Cell Proliferation. In Anticancer research, 39, 6007-6014. doi:10.21873/anticanres.13806. https://pubmed.ncbi.nlm.nih.gov/31704826/
8. Yu, Weijia, Lutz, Christoph, Krämer, Alwin, Schmidt-Zachmann, Marion S. 2021. The JmjC-domain protein NO66/RIOX-1 affects the balance between proliferation and maturation in acute myeloid leukemia. In Experimental cell research, 402, 112566. doi:10.1016/j.yexcr.2021.112566. https://pubmed.ncbi.nlm.nih.gov/33745927/
9. Wang, Chengliang, Zhang, Qiongdi, Hang, Tianrong, Zhang, Xuan, Zang, Jianye. 2015. Structure of the JmjC domain-containing protein NO66 complexed with ribosomal protein Rpl8. In Acta crystallographica. Section D, Biological crystallography, 71, 1955-64. doi:10.1107/S1399004715012948. https://pubmed.ncbi.nlm.nih.gov/26327385/
10. Sinha, Krishna M, Bagheri-Yarmand, Rozita, Lahiri, Sharmistha, Gagel, Robert F, Huard, Johnny. 2019. Oncogenic and osteolytic functions of histone demethylase NO66 in castration-resistant prostate cancer. In Oncogene, 38, 5038-5049. doi:10.1038/s41388-019-0774-x. https://pubmed.ncbi.nlm.nih.gov/30858546/