Cysltr2-KO 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Cysltr2-KO 基因敲除小鼠

产品编号

S-KO-13256

品系全称

C57BL/6JCya-Cysltr2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-70086-Cysltr2-B6J-VA

品系状态

使用本品系发表的文献需注明: Cysltr2-KO 基因敲除小鼠 mice (Strain S-KO-13256) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
cysteinyl leukotriene receptor 2
基因别称
2300001H05Rik,CYSLT2R,Cltr2,Cyslt2
染色体号
Chr 14 (Mouse)
转录本 ID
NCBI: NM_133720 | Ensembl: ENSMUST00000044664
修饰方式
全身性基因敲除
靶向范围
Exon 6
敲除长度
~2.7 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1917336Homozygous null mice display reduced bleomycin-induced pulmonary fibrosis and reduced IgE dependent passive cutaneous anaphylaxis.
CYSLTR2,也称为Cysteinyl Leukotriene Receptor 2,是一种编码G蛋白偶联受体(GPCR)的基因。CYSLTR2编码的受体,即CysLTR2,属于GPCR家族,是一种细胞表面受体,能够与半胱氨酸白三烯(CysLTs)结合,并激活下游信号通路。CysLTs是一类具有多种生物活性的脂质介质,参与炎症反应、免疫调节和细胞增殖等多种生理和病理过程。CYSLTR2在多种组织中表达,包括免疫细胞、肺、皮肤和眼睛等。

CYSLTR2在多种疾病中发挥重要作用,包括哮喘、过敏、炎症性肠病、癌症和骨代谢疾病。例如,CYSLTR2基因的多态性与哮喘、过敏和寄生虫感染的风险相关[3]。CYSLTR2在结直肠癌中的表达和甲基化状态与患者的预后和转移风险相关[2]。CYSLTR2在骨代谢疾病中的表达与骨密度的降低和骨折风险的增加相关[6]。

在癌症方面,CYSLTR2基因的突变与色素性上皮样细胞瘤(PEM)、蓝痣和脉络膜黑色素瘤的发生和进展相关。例如,CYSLTR2基因的L129Q突变是蓝痣和脉络膜黑色素瘤的一种常见突变,能够导致受体的持续激活和信号通路失调,从而促进肿瘤的发生和进展[4][5]。此外,CYSLTR2基因的L129Q突变还能够逃避免疫细胞的抑制,进一步促进肿瘤的生长和扩散[1]。

CYSLTR2基因的突变和表达调控机制复杂,涉及到多种信号通路和调节因子。例如,CYSLTR2基因的表达受到转录因子和表观遗传调控的影响,包括DNA甲基化和组蛋白修饰等[2]。CYSLTR2基因的突变还能够影响受体的内吞和降解过程,从而影响受体的稳定性和功能[1]。

综上所述,CYSLTR2基因在多种生理和病理过程中发挥重要作用,其突变和表达调控机制复杂,涉及到多种信号通路和调节因子。CYSLTR2基因的研究有助于深入理解GPCR的功能和调控机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Ceraudo, Emilie, Horioka, Mizuho, Mattheisen, Jordan M, Sakmar, Thomas P, Huber, Thomas. 2020. Direct evidence that the GPCR CysLTR2 mutant causative of uveal melanoma is constitutively active with highly biased signaling. In The Journal of biological chemistry, 296, 100163. doi:10.1074/jbc.RA120.015352. https://pubmed.ncbi.nlm.nih.gov/33288675/
2. Ghatak, Souvik, Satapathy, Shakti Ranjan, Sjölander, Anita. 2023. DNA Methylation and Gene Expression of the Cysteinyl Leukotriene Receptors as a Prognostic and Metastatic Factor for Colorectal Cancer Patients. In International journal of molecular sciences, 24, . doi:10.3390/ijms24043409. https://pubmed.ncbi.nlm.nih.gov/36834820/
3. Dos S Jesus, Talita, Dos S Costa, Ryan, Alcântara-Neves, Neuza Maria, Barreto, Maurício L, Figueiredo, Camila Alexandrina. 2019. Variants in the CYSLTR2 are associated with asthma, atopy markers and helminths infections in the Brazilian population. In Prostaglandins, leukotrienes, and essential fatty acids, 145, 15-22. doi:10.1016/j.plefa.2019.05.003. https://pubmed.ncbi.nlm.nih.gov/31126515/
4. Möller, Inga, Murali, Rajmohan, Müller, Hansgeorg, Schadendorf, Dirk, Griewank, Klaus G. 2016. Activating cysteinyl leukotriene receptor 2 (CYSLTR2) mutations in blue nevi. In Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 30, 350-356. doi:10.1038/modpathol.2016.201. https://pubmed.ncbi.nlm.nih.gov/27934878/
5. de la Fouchardiere, Arnaud, Tirode, Franck, Castillo, Christine, Macagno, Nicolas, Pissaloux, Daniel. 2022. Attempting to Solve the Pigmented Epithelioid Melanocytoma (PEM) Conundrum: PRKAR1A Inactivation Can Occur in Different Genetic Backgrounds (Common, Blue, and Spitz Subgroups) With Variation in Their Clinicopathologic Characteristics. In The American journal of surgical pathology, 46, 1106-1115. doi:10.1097/PAS.0000000000001888. https://pubmed.ncbi.nlm.nih.gov/35319526/
6. Fang, Shenyun, Ni, Haonan, Zhang, Qianghua, Zhang, Weili, Li, Haidong. 2024. Integrated single-cell and bulk RNA sequencing analysis reveal immune-related biomarkers in postmenopausal osteoporosis. In Heliyon, 10, e38022. doi:10.1016/j.heliyon.2024.e38022. https://pubmed.ncbi.nlm.nih.gov/39328516/