Neat1-KO 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Neat1-KO 基因敲除小鼠

产品编号

S-KO-12018

品系全称

C57BL/6JCya-Neat1em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-66961-Neat1-B6J-VA

品系状态

使用本品系发表的文献需注明: Neat1-KO 基因敲除小鼠 mice (Strain S-KO-12018) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
nuclear paraspeckle assembly transcript 1 (non-protein coding)
基因别称
2310043N10Rik,VINC
染色体号
Chr 19 (Mouse)
转录本 ID
NCBI: NR_131212 | Ensembl: ENSMUST00000173672
修饰方式
全身性基因敲除
靶向范围
Exon 1
敲除长度
~21.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1914211Mice homozygous for a null mutation lack paraspeckles in the nucleus and exhibit reduced pup survival associated with impaired mammary gland development.
NEAT1,全称为核旁斑点组装转录本1,是一种长链非编码RNA(lncRNA)。lncRNA是一类长度超过200个核苷酸的RNA分子,它们不编码蛋白质,但参与了多种生物学过程,包括基因表达调控、细胞分化、发育和疾病发生。NEAT1在多种哺乳动物细胞类型中广泛表达,并在多种生物和病理过程中发挥关键作用。

NEAT1在多种生物和病理过程中发挥着关键作用。例如,有研究表明,NEAT1在疱疹单纯病毒1型感染和阿尔茨海默病中,通过表观遗传调控下游基因转录,发挥着重要的传感器和效应器作用。此外,NEAT1还在神经胶质细胞中通过表观遗传调控自噬相关基因的表达,促进自噬的发生。在神经损伤引起的神经病理性疼痛中,NEAT1通过调节背根神经节中促炎基因的表达,参与神经免疫通信的调节。

在癌症研究中,NEAT1也显示出了重要的功能。例如,在胶质瘤中,NEAT1通过稳定PGK1,促进胶质瘤的进展。在多发性骨髓瘤中,NEAT1的高表达与不良的治疗反应和疾病的进展相关。在胃癌中,NEAT1通过介导RPRD1B的稳定性,促进脂肪酸代谢和淋巴结转移。此外,有研究表明,MUC1-C亚基在癌症进展中通过调节NEAT1的表达和旁斑形成发挥着重要作用。

综上所述,NEAT1是一种功能多样的lncRNA,它在多种生物学过程中发挥着关键作用。NEAT1的研究有助于深入理解lncRNA的功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略[1,2,3,4,5,6,7,8,9,10]。

参考文献:
1. Wang, Ziqiang, Li, Kun, Huang, Weiren. 2020. Long non-coding RNA NEAT1-centric gene regulation. In Cellular and molecular life sciences : CMLS, 77, 3769-3779. doi:10.1007/s00018-020-03503-0. https://pubmed.ncbi.nlm.nih.gov/32219465/
2. Liang, Jingshan, Liu, Changtao, Xu, Dezhi, Xie, Kang, Li, Aimin. 2022. LncRNA NEAT1 facilitates glioma progression via stabilizing PGK1. In Journal of translational medicine, 20, 80. doi:10.1186/s12967-022-03273-2. https://pubmed.ncbi.nlm.nih.gov/35123484/
3. Fierro, Claudia, Gatti, Veronica, La Banca, Veronica, Melino, Gerry, Peschiaroli, Angelo. 2023. The long non-coding RNA NEAT1 is a ΔNp63 target gene modulating epidermal differentiation. In Nature communications, 14, 3795. doi:10.1038/s41467-023-39011-5. https://pubmed.ncbi.nlm.nih.gov/37365156/
4. Wen, Simeng, Wei, Yulei, Zen, Chong, Niu, Yuanjie, Zhao, Yu. 2020. Long non-coding RNA NEAT1 promotes bone metastasis of prostate cancer through N6-methyladenosine. In Molecular cancer, 19, 171. doi:10.1186/s12943-020-01293-4. https://pubmed.ncbi.nlm.nih.gov/33308223/
5. Maruyama, Motoyo, Sakai, Atsushi, Fukunaga, Tsukasa, Hamada, Michiaki, Suzuki, Hidenori. 2023. Neat1 lncRNA organizes the inflammatory gene expressions in the dorsal root ganglion in neuropathic pain caused by nerve injury. In Frontiers in immunology, 14, 1185322. doi:10.3389/fimmu.2023.1185322. https://pubmed.ncbi.nlm.nih.gov/37614230/
6. Chen, Ting, Sun, Zhengxu, Cui, Yunqi, Li, Yating, Qu, Xiaoyan. . Identification of long noncoding RNA NEAT1 as a key gene involved in the extramedullary disease of multiple myeloma by bioinformatics analysis. In Hematology (Amsterdam, Netherlands), 28, 2164449. doi:10.1080/16078454.2022.2164449. https://pubmed.ncbi.nlm.nih.gov/36657019/
7. Wang, Ziqiang, Zhang, Shikuan, Li, Kun. 2021. LncRNA NEAT1 induces autophagy through epigenetic regulation of autophagy-related gene expression in neuroglial cells. In Journal of cellular physiology, 237, 824-832. doi:10.1002/jcp.30556. https://pubmed.ncbi.nlm.nih.gov/34402054/
8. Bhattacharya, Atrayee, Wang, Keyi, Penailillo, Johany, Miyo, Masaaki, Kufe, Donald W. 2024. MUC1-C regulates NEAT1 lncRNA expression and paraspeckle formation in cancer progression. In Oncogene, 43, 2199-2214. doi:10.1038/s41388-024-03068-3. https://pubmed.ncbi.nlm.nih.gov/38802648/
9. Jia, Yongxu, Yan, Qian, Zheng, Yinli, Qin, Yanru, Guan, Xin-Yuan. 2022. Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer. In Journal of experimental & clinical cancer research : CR, 41, 287. doi:10.1186/s13046-022-02449-4. https://pubmed.ncbi.nlm.nih.gov/36171622/
10. Haghighi, Nastaran, Doosti, Abbas, Kiani, Jafar. 2021. Evaluation of CRISPR/Cas9 System Effects on Knocking Out NEAT1 Gene in AGS Gastric Cancer Cell Line with Therapeutic Perspective. In Journal of gastrointestinal cancer, 53, 623-631. doi:10.1007/s12029-021-00669-z. https://pubmed.ncbi.nlm.nih.gov/34357544/