Rbm46-KO 基因敲除小鼠

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产品名称

Rbm46-KO 基因敲除小鼠

产品编号

S-KO-11350

品系全称

C57BL/6NCya-Rbm46em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-633285-Rbm46-B6N-VA

品系状态

使用本品系发表的文献需注明: Rbm46-KO 基因敲除小鼠 mice (Strain S-KO-11350) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
RNA binding motif protein 46
基因别称
EG633285,Gm761
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_001277170 | Ensembl: ENSMUST00000182818
修饰方式
全身性基因敲除
靶向范围
Exon 2~3
敲除长度
~2.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:3645057Mice with a homozygous frameshifting deletion between exons 2-3 exhibit infertility, with absence of oocytes, small testes and seminiferous tubules containing only Sertoli cells and few undifferentiated spermatogonia.
基因RBM46,也称为RNA结合蛋白46,是一种在哺乳动物生殖细胞中特异表达的RNA结合蛋白。RBM46在精子发生过程中发挥着关键作用,它参与调控基因表达,尤其是在从有丝分裂到减数分裂的转变过程中[1][2][3][4]。研究表明,RBM46通过与YTHDC2和MEIOC形成复合物,共同调控转录后水平的基因表达[1][2][5]。RBM46可以结合到mRNA的3'非翻译区(3'UTR),并通过促进mRNA的降解或抑制其翻译来调控基因表达[1][2][3][4]。此外,RBM46还在胚胎干细胞(ESC)的分化过程中发挥重要作用,它可以结合并降解β-Catenin mRNA,从而影响Wnt/β-Catenin信号通路[7]。

RBM46的异常表达与男性生育能力障碍相关[1][3]。例如,在人类男性不育症中,RBM46的表达异常与精子发生障碍相关[1]。此外,RBM46的突变或缺失会导致小鼠和小鱼的不育[3][4]。RBM46的缺失还会导致精子发生过程中减数分裂复合物的形成异常,从而导致精子发生障碍[2]。

除了在生殖细胞中的作用外,RBM46还与癌症的发生发展相关。例如,RBM46在膀胱癌患者的尿液中表达上调,可能与膀胱癌的发生发展相关[6]。此外,RBM46在胶质母细胞瘤中表达上调,并且与不良预后相关[8]。

综上所述,RBM46是一种在生殖细胞中特异表达的RNA结合蛋白,它参与调控转录后水平的基因表达,影响精子发生和胚胎干细胞分化。RBM46的异常表达与男性生育能力障碍和癌症的发生发展相关。进一步研究RBM46的功能和调控机制,有助于深入理解生殖细胞分化和癌症发生发展的分子机制,为相关疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Qian, Baomei, Li, Yang, Yan, Ruoyu, Zhou, Jian, Ye, Lan. 2022. RNA binding protein RBM46 regulates mitotic-to-meiotic transition in spermatogenesis. In Science advances, 8, eabq2945. doi:10.1126/sciadv.abq2945. https://pubmed.ncbi.nlm.nih.gov/36001654/
2. Lv, Yue, Lu, Gang, Cai, Yuling, Liu, Hongbin, Chan, Wai-Yee. 2022. RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits. In Protein & cell, 14, 51-63. doi:10.1093/procel/pwac040. https://pubmed.ncbi.nlm.nih.gov/36726756/
3. Peart, Natoya J, Johnson, Taylor A, Lee, Sungkyoung, Geyer, Christopher B, Carstens, Russ P. 2022. The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. In PLoS genetics, 18, e1010416. doi:10.1371/journal.pgen.1010416. https://pubmed.ncbi.nlm.nih.gov/36129965/
4. Dai, Xiangyan, Cheng, Xinkai, Huang, Jianfei, Wang, Zhijian, Yin, Zhan. . Rbm46, a novel germ cell-specific factor, modulates meiotic progression and spermatogenesis. In Biology of reproduction, 104, 1139-1153. doi:10.1093/biolre/ioab016. https://pubmed.ncbi.nlm.nih.gov/33524105/
5. Pfaltzgraff, Natalie G, Liu, Bingrun, de Rooij, Dirk G, Page, David C, Mikedis, Maria M. 2024. Destabilization of mRNAs enhances competence to initiate meiosis in mouse spermatogenic cells. In Development (Cambridge, England), 151, . doi:10.1242/dev.202740. https://pubmed.ncbi.nlm.nih.gov/38884383/
6. Yazarlou, Fatemeh, Kholghi-Oskooei, Vahid, Afsharpad, Mandana, Ghafouri-Fard, Soudeh, Modarressi, Mohammad H. 2018. Expression analysis of a panel of cancer-testis antigens in bladder cancer. In Personalized medicine, 15, 511-520. doi:10.2217/pme-2018-0049. https://pubmed.ncbi.nlm.nih.gov/30362892/
7. Zhai, Lei, Wang, Chenchen, Chen, Yuanfan, Zhou, Shixin, Li, Lingsong. 2017. Rbm46 regulates mouse embryonic stem cell differentiation by targeting β-Catenin mRNA for degradation. In PloS one, 12, e0172420. doi:10.1371/journal.pone.0172420. https://pubmed.ncbi.nlm.nih.gov/28212427/
8. Xing, Yi-Qi, Zhu, Ting-Zhun. . RNA-Binding Motif Protein RBM47 Promotes Invasiveness of Glioblastoma Through Activation of Epithelial-to-Mesenchymal Transition Program. In Genetic testing and molecular biomarkers, 27, 384-392. doi:10.1089/gtmb.2023.0368. https://pubmed.ncbi.nlm.nih.gov/38156907/