Tff2-KO 基因敲除小鼠

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产品名称

Tff2-KO 基因敲除小鼠

产品编号

S-KO-05350

品系全称

C57BL/6JCya-Tff2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-21785-Tff2-B6J-VA

品系状态

使用本品系发表的文献需注明: Tff2-KO 基因敲除小鼠 mice (Strain S-KO-05350) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
trefoil factor 2 (spasmolytic protein 1)
基因别称
SP,mSP
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_009363 | Ensembl: ENSMUST00000024826
修饰方式
全身性基因敲除
靶向范围
Exon 1~4
敲除长度
~5.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1306805Homozygous mutation of this gene results in decreased gastric proliferation, increased acid secretion, increased susceptibility to gastric ulceration after indomethacin administration, and altered regulation of genes involved in adaptive and immune responses in the pyloric antrum.
Tff2,也称为Spasmolytic polypeptide或Trefoil factor 2,是一种分泌性蛋白质,主要在胃粘膜的粘液细胞中表达。Tff2具有抗炎和保护胃粘膜的作用,通过促进细胞粘附、迁移和增殖,参与胃粘膜的修复和再生。Tff2的表达受多种因素调控,包括炎症、氧化应激和细胞因子等。

Tff2在胃癌的发生和发展中也起着重要作用。有研究表明,Tff2的表达与胃癌的分化程度和预后相关,低分化胃癌中Tff2的表达水平较低,预后较差。此外,Tff2的表达还与幽门螺杆菌感染和MUC1的表达相关,提示Tff2可能参与了幽门螺杆菌感染和MUC1介导的胃癌发生过程。

在胃粘膜的修复和再生过程中,Tff2的表达受到多种因素的调控。研究表明,炎症和M2巨噬细胞浸润在Tff2表达的诱导中起着重要作用。炎症和M2巨噬细胞通过分泌细胞因子和生长因子,促进Tff2的表达和胃粘膜的修复。此外,Tff2的表达还受到其自身表达的调控,Tff2能够通过激活其自身启动子,促进其表达,形成正反馈循环,进一步促进胃粘膜的修复和再生。

Tff2的表达和功能还受到其他基因的调控。有研究表明,MUC1的表达与Tff2的甲基化水平相关,MUC1的表达能够降低Tff2的甲基化水平,从而促进Tff2的表达。此外,Tff2的表达还受到其他基因的调控,例如CD44和Fcgbp等,这些基因的表达与Tff2的表达和功能密切相关。

综上所述,Tff2是一种重要的分泌性蛋白质,在胃粘膜的修复和再生中起着重要作用。Tff2的表达和功能受到多种因素的调控,包括炎症、氧化应激、细胞因子、自身表达和其他基因的调控。Tff2的研究有助于深入理解胃粘膜的修复和再生机制,为胃癌的治疗和预防提供新的思路和策略[1][2][3][4][5][6][7][8][9][10]。

参考文献:
1. Teal, Emma, Dua-Awereh, Martha, Hirshorn, Sabrina T, Zavros, Yana. 2020. Role of metaplasia during gastric regeneration. In American journal of physiology. Cell physiology, 319, C947-C954. doi:10.1152/ajpcell.00415.2019. https://pubmed.ncbi.nlm.nih.gov/32755448/
2. Zhang, Min, Hu, Shuofeng, Min, Min, Ying, Xiaomin, Liu, Yan. 2020. Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing. In Gut, 70, 464-475. doi:10.1136/gutjnl-2019-320368. https://pubmed.ncbi.nlm.nih.gov/32532891/
3. Kim, Young S, Ho, Samuel B. . Intestinal goblet cells and mucins in health and disease: recent insights and progress. In Current gastroenterology reports, 12, 319-30. doi:10.1007/s11894-010-0131-2. https://pubmed.ncbi.nlm.nih.gov/20703838/
4. Thiem, Stefan, Eissmann, Moritz F, Stuart, Emma, Buchert, Michael, Ernst, Matthias. 2016. Inducible gene modification in the gastric epithelium of Tff1-CreERT2, Tff2-rtTA, Tff3-luc mice. In Genesis (New York, N.Y. : 2000), 54, 626-635. doi:10.1002/dvg.22987. https://pubmed.ncbi.nlm.nih.gov/27731922/
5. Dubeykovskaya, Zinaida A, Duddempudi, Phaneendra Kumar, Deng, Huan, Kitajewski, Jan, Wang, Timothy C. 2018. Therapeutic potential of adenovirus-mediated TFF2-CTP-Flag peptide for treatment of colorectal cancer. In Cancer gene therapy, 26, 48-57. doi:10.1038/s41417-018-0036-z. https://pubmed.ncbi.nlm.nih.gov/30042499/
6. Ge, Yuqiu, Ma, Gaoxiang, Liu, Hanting, Zhang, Haiyan, Zhang, Zhengdong. 2020. MUC1 is associated with TFF2 methylation in gastric cancer. In Clinical epigenetics, 12, 37. doi:10.1186/s13148-020-00832-6. https://pubmed.ncbi.nlm.nih.gov/32122390/
7. Bockerstett, Kevin A, Lewis, Scott A, Wolf, Kyle J, Ahn, Tae-Hyuk, DiPaolo, Richard J. 2019. Single-cell transcriptional analyses of spasmolytic polypeptide-expressing metaplasia arising from acute drug injury and chronic inflammation in the stomach. In Gut, 69, 1027-1038. doi:10.1136/gutjnl-2019-318930. https://pubmed.ncbi.nlm.nih.gov/31481545/
8. Hirata, Koji, Kodama, Sota, Nakano, Yasuhiro, Uemoto, Shinji, Kawaguchi, Yoshiya. 2019. Exocrine tissue-driven TFF2 prevents apoptotic cell death of endocrine lineage during pancreas organogenesis. In Scientific reports, 9, 1636. doi:10.1038/s41598-018-38062-9. https://pubmed.ncbi.nlm.nih.gov/30733468/
9. Zhu, Zoe Xiaofang, Liu, Yao, Wang, Jinghao, Jeong, Y Natalie, Chen, Jake Jinkun. 2023. A novel lncRNA-mediated epigenetic regulatory mechanism in periodontitis. In International journal of biological sciences, 19, 5187-5203. doi:10.7150/ijbs.87977. https://pubmed.ncbi.nlm.nih.gov/37928259/
10. Bulitta, Clemens J, Fleming, John V, Raychowdhury, Raktima, Rosenberg, Ian, Wang, Timothy C. . Autoinduction of the trefoil factor 2 (TFF2) promoter requires an upstream cis-acting element. In Biochemical and biophysical research communications, 293, 366-74. doi:. https://pubmed.ncbi.nlm.nih.gov/12054609/