Nr1d1-KO 基因敲除小鼠

下单100%中奖,最高可得千元京东卡
复苏/繁育服务
产品名称

Nr1d1-KO 基因敲除小鼠

产品编号

S-KO-05255

品系全称

C57BL/6NCya-Nr1d1em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-217166-Nr1d1-B6N-VA

品系状态

使用本品系发表的文献需注明: Nr1d1-KO 基因敲除小鼠 mice (Strain S-KO-05255) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
KO小鼠库模型
核受体Brite

基本信息

基因研究概述

质控标准

基因
基因全称
nuclear receptor subfamily 1, group D, member 1
基因别称
A530070C09Rik
染色体号
Chr 11 (Mouse)
转录本 ID
NCBI: NM_145434 | Ensembl: ENSMUST00000064941
修饰方式
全身性基因敲除
靶向范围
Exon 2~5
敲除长度
~1.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2444210Homozygotes for targeted null mutations exhibit altered circadian behavior and temperature regulation with mild alterations in cytoarchitecture of the cerebellum. Litter size is reduced by half in mutant females.

发表文献

Science Bulletin
2021-11-15
Single-cell RNA-seq and chromatin accessibility profiling decipher the heter‐ogeneity of mouse γ δ T cells
Hepatology
2019
Reverse Erythroblastosis Virus α Antagonism Promotes Homocysteine Catabolism and Ammonia Clearance.
1
NR1D1,也称为REV-ERBα,属于核受体(NR)家族,是一种重要的转录因子,在多种生理和病理过程中发挥重要作用。NR1D1与血红素结合,是昼夜节律钟的核心组成部分,参与调控多种生物学过程,包括细胞代谢、炎症反应、免疫和衰老等。

NR1D1在多种器官中发挥重要作用。例如,在血管平滑肌细胞(VSMC)中,NR1D1通过靶向线粒体三羧酸循环(TCA)的关键酶 aconitase-2(ACO2)来调节细胞代谢,影响腹部主动脉瘤(AAA)的形成[1]。在RA患者中,NR1D1表达增加,激活NR1D1可以减少炎症反应和骨破坏,抑制RA的发展[3]。在膀胱癌(BC)患者中,NR1D1表达水平与临床特征和预后相关,NR1D1可能成为治疗BC的潜在靶点[2]。

NR1D1的调控机制复杂多样。例如,m6A甲基化可以导致NR1D1降解,从而影响其功能。在肝纤维化模型中,NR1D1的降解导致线粒体功能障碍和炎症反应,加剧肝纤维化的进展[4]。此外,NR1D1还可以通过抑制Hmga2的转录来减轻视网膜炎症[5]。

NR1D1的研究不仅有助于深入理解昼夜节律钟的生物学功能和疾病发生机制,还为疾病的治疗和预防提供了新的思路和策略。例如,α-酮戊二酸(αKG)可以恢复NR1D1缺乏引起的线粒体功能障碍,预防AAA的发生[1]。NR1D1激动剂SR9009可以减轻视网膜炎症、哮喘和溃疡性结肠炎的症状[5,6,7]。这些研究结果表明,NR1D1及其激动剂可能成为多种疾病的治疗靶点。

参考文献:
1. Sun, Ling-Yue, Lyu, Yu-Yan, Zhang, Heng-Yuan, Qian, Kun, Pu, Jun. 2022. Nuclear Receptor NR1D1 Regulates Abdominal Aortic Aneurysm Development by Targeting the Mitochondrial Tricarboxylic Acid Cycle Enzyme Aconitase-2. In Circulation, 146, 1591-1609. doi:10.1161/CIRCULATIONAHA.121.057623. https://pubmed.ncbi.nlm.nih.gov/35880522/
2. Yang, Yubo, Bai, Yunjin, Wang, Xiaoming, Li, Dengxiong, Han, Ping. 2023. Clock gene NR1D1 might be a novel target for the treatment of bladder cancer. In Urologic oncology, 41, 327.e9-327.e18. doi:10.1016/j.urolonc.2023.04.021. https://pubmed.ncbi.nlm.nih.gov/37208228/
3. Liu, Hui, Zhu, Yuanli, Gao, Yutong, Guo, Fengjing, Xiao, Jun. 2020. NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis. In Cell death & disease, 11, 129. doi:10.1038/s41419-020-2314-6. https://pubmed.ncbi.nlm.nih.gov/32071294/
4. Chen, Li, Xia, Siwei, Wang, Feixia, Zhang, Feng, Zheng, Shizhong. 2023. m6A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis. In Pharmacological research, 189, 106704. doi:10.1016/j.phrs.2023.106704. https://pubmed.ncbi.nlm.nih.gov/36813093/
5. Wang, Zhijie, Huang, Yinhua, Chu, Feixue, Chen, Jiansu, Tang, Shibo. 2021. Clock Gene Nr1d1 Alleviates Retinal Inflammation Through Repression of Hmga2 in Microglia. In Journal of inflammation research, 14, 5901-5918. doi:10.2147/JIR.S326091. https://pubmed.ncbi.nlm.nih.gov/34795498/
6. Tiwari, Drishti, Ahuja, Nancy, Kumar, Sumit, Arora, Rashmi, Gupta, Pawan. 2022. Nuclear receptor Nr1d1 alleviates asthma by abating GATA3 gene expression and Th2 cell differentiation. In Cellular and molecular life sciences : CMLS, 79, 308. doi:10.1007/s00018-022-04323-0. https://pubmed.ncbi.nlm.nih.gov/35596832/
7. Chen, Yidong, Li, Junrong, Li, Shuang, Li, Jiamin, Zhu, Liangru. 2023. Uncovering the Novel Role of NR1D1 in Regulating BNIP3-Mediated Mitophagy in Ulcerative Colitis. In International journal of molecular sciences, 24, . doi:10.3390/ijms241814222. https://pubmed.ncbi.nlm.nih.gov/37762536/

发表文献

点击查看发表文献 >>