St8sia1-KO 基因敲除小鼠

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产品名称

St8sia1-KO 基因敲除小鼠

产品编号

S-KO-04317

品系全称

C57BL/6NCya-St8sia1em1/Cya

品系背景

C57BL/6NCya

品系编号

KOCMP-20449-St8sia1-B6N-VA

品系状态

使用本品系发表的文献需注明: St8sia1-KO 基因敲除小鼠 mice (Strain S-KO-04317) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
基因别称
9330109E03Rik,GD3S,Sia-T,Siat8,Siat8a
染色体号
Chr 6 (Mouse)
转录本 ID
NCBI: NM_011374 | Ensembl: ENSMUST00000032421
修饰方式
全身性基因敲除
靶向范围
Exon 2
敲除长度
~1.1 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:106011Homozygotes for a targeted allele are behaviorally normal with no signs of aberrant brain histology or demyelination. Homozygotes for a knock-out allele are behaviorally intact with normal nervous tissue morphology and sensitivity to Fas-mediated apoptosis but show impaired repair of damaged nerves.
ST8SIA1,也称为α-2,8-唾液酸转移酶1,是一种重要的唾液酸转移酶。唾液酸转移酶是一类负责在糖链末端添加唾液酸残基的酶,参与合成复杂的糖链,如糖脂和糖蛋白,这些糖链在细胞识别、信号转导和免疫应答等生物学过程中发挥重要作用。ST8SIA1在多种细胞类型中表达,包括免疫细胞和肿瘤细胞,其表达水平与多种疾病的发生和发展密切相关。

ST8SIA1在类风湿性关节炎(RA)的发生和发展中发挥重要作用。一项研究表明,ST8SIA1是RA诊断的核心基因之一,与RA的发病机制密切相关[1]。此外,ST8SIA1还与胰腺癌、肾细胞癌、乳腺癌、神经母细胞瘤和结直肠癌等多种癌症的发生和发展密切相关。例如,在肾细胞癌中,ST8SIA1表达上调,与患者的预后不良相关[2]。在乳腺癌中,ST8SIA1表达上调,与患者的不良生存率相关[3]。在神经母细胞瘤中,ST8SIA1表达下调,与患者的抗GD2免疫治疗耐药性相关[4]。在结直肠癌中,ST8SIA1表达上调,与患者的预后不良相关[5]。

ST8SIA1的表达受多种因素调控。例如,雌激素可以抑制ST8SIA1的表达[6]。DNA甲基化也可以调节ST8SIA1的表达,例如,在胶质瘤细胞中,ST8SIA1基因启动子区域的DNA甲基化水平与其表达水平相关[7]。

ST8SIA1的功能机制也受到广泛关注。例如,ST8SIA1可以激活FAK-AKT-mTOR信号通路,促进乳腺癌的生长和转移[8]。ST8SIA1还可以通过阻断JAK/STAT信号通路抑制膀胱癌细胞的增殖、迁移和侵袭[9]。

综上所述,ST8SIA1是一种重要的唾液酸转移酶,参与合成复杂的糖链,在细胞识别、信号转导和免疫应答等生物学过程中发挥重要作用。ST8SIA1在多种疾病的发生和发展中发挥重要作用,包括RA、胰腺癌、肾细胞癌、乳腺癌、神经母细胞瘤和结直肠癌等。ST8SIA1的表达受多种因素调控,其功能机制也受到广泛关注。ST8SIA1的研究有助于深入理解糖链的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Zhao, ZhenYu, He, ShaoJie, Yu, XinCheng, Zeng, Shan, Zha, DingSheng. 2022. Analysis and Experimental Validation of Rheumatoid Arthritis Innate Immunity Gene CYFIP2 and Pan-Cancer. In Frontiers in immunology, 13, 954848. doi:10.3389/fimmu.2022.954848. https://pubmed.ncbi.nlm.nih.gov/35898498/
2. Hu, Xu, Yang, Yanfei, Wang, Yaohui, Ren, Shangqing, Li, Xiang. 2022. Identifying an Immune-Related Gene ST8SIA1 as a Novel Target in Patients With Clear-Cell Renal Cell Carcinoma. In Frontiers in pharmacology, 13, 901518. doi:10.3389/fphar.2022.901518. https://pubmed.ncbi.nlm.nih.gov/35873547/
3. Kan, Jung-Yu, Moi, Sin-Hua, Hung, Wen-Chun, Li, Chung-Liang, Chiang, Chih-Po. 2020. Comprehensive Transcriptomic Analysis Identifies ST8SIA1 as a Survival-Related Sialyltransferase Gene in Breast Cancer. In Genes, 11, . doi:10.3390/genes11121436. https://pubmed.ncbi.nlm.nih.gov/33260650/
4. Chopra, A, Mueller, R, Weiner, J, Grohmann, E, Schaefer, A S. 2021. BACH1 Binding Links the Genetic Risk for Severe Periodontitis with ST8SIA1. In Journal of dental research, 101, 93-101. doi:10.1177/00220345211017510. https://pubmed.ncbi.nlm.nih.gov/34160287/
5. Zhang, Chang, Wang, Yeli, Yu, Yao, Xiao, Xiao, Hao, Leilei. 2024. Overexpression of ST8Sia1 inhibits tumor progression by TGF-β1 signaling in rectal adenocarcinoma and promotes the tumoricidal effects of CD8+ T cells by granzyme B and perforin. In Annals of medicine, 57, 2439539. doi:10.1080/07853890.2024.2439539. https://pubmed.ncbi.nlm.nih.gov/39656552/
6. Bobowski, Marie, Vincent, Audrey, Steenackers, Agata, Julien, Sylvain, Delannoy, Philippe. 2013. Estradiol represses the G(D3) synthase gene ST8SIA1 expression in human breast cancer cells by preventing NFκB binding to ST8SIA1 promoter. In PloS one, 8, e62559. doi:10.1371/journal.pone.0062559. https://pubmed.ncbi.nlm.nih.gov/23626833/
7. Yamamoto, Yurie, Higashimoto, Ken, Ohkawa, Yuki, Furukawa, Keiko, Furukawa, Koichi. 2023. Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells. In Glycoconjugate journal, 40, 323-332. doi:10.1007/s10719-023-10108-9. https://pubmed.ncbi.nlm.nih.gov/36897478/
8. Nguyen, Khoa, Yan, Yuanqing, Yuan, Bin, Andreeff, Michael, Battula, V Lokesh. 2018. ST8SIA1 Regulates Tumor Growth and Metastasis in TNBC by Activating the FAK-AKT-mTOR Signaling Pathway. In Molecular cancer therapeutics, 17, 2689-2701. doi:10.1158/1535-7163.MCT-18-0399. https://pubmed.ncbi.nlm.nih.gov/30237308/
9. Yu, Shengjin, Wang, Shidan, Sun, Xiaoxin, Yang, Deyong, Jiang, Yu. 2021. ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway. In Oncology letters, 22, 736. doi:10.3892/ol.2021.12997. https://pubmed.ncbi.nlm.nih.gov/34429775/