Tpsb2-KO 基因敲除小鼠

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产品名称

Tpsb2-KO 基因敲除小鼠

产品编号

S-KO-03163

品系全称

C57BL/6JCya-Tpsb2em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-17229-Tpsb2-B6J-VA

品系状态

使用本品系发表的文献需注明: Tpsb2-KO 基因敲除小鼠 mice (Strain S-KO-03163) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
tryptase beta 2
基因别称
MMCP-6,Mcp-6,Mcp6,Mcpt6
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_010781 | Ensembl: ENSMUST00000234477
修饰方式
全身性基因敲除
靶向范围
Exon 3
敲除长度
~0.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:96942Mice homozygous for a knock-out allele show reduced recruitment of eosinophils to sites of T. spiralis larvae and decreased elimination of larvae in chronically infected skeletal muscle. Mice homozygous for a knock-in allele show a reduced ability to clear K. pneumoniae from their peritoneal cavity.
Tpsb2,也称为tryptase beta 2,是一种编码β-tryptase的基因。β-tryptase是一种丝氨酸蛋白酶,主要由肥大细胞产生,并存在于所有人类肥大细胞的分泌颗粒中。β-tryptase在多种生物学过程中发挥重要作用,包括炎症反应、过敏性疾病、肿瘤发生和发展等。

Tpsb2基因的表达受到多种转录因子的调控,包括GATA1和GATA2。研究表明,在骨髓来源的肥大细胞中,GATA1和GATA2的减少会导致Tpsb2和Tpsg1基因的表达显著下降。此外,CTCF和cohesin亚基Rad21的绑定也被发现与Tpsb2和Tpsg1基因的表达相关[7]。

β-tryptase具有多种生物学功能,包括降解多种蛋白质,如纤维蛋白原、纤连蛋白、尿激酶型纤溶酶原激活剂前体、基质金属蛋白酶-3前体、蛋白酶激活受体-2和补体成分C3等。β-tryptase还可以激活多种细胞因子和趋化因子,如IL-1β、TNF-α、IL-6等,参与炎症反应和过敏性疾病的发生和发展[10]。

研究表明,Tpsb2基因的表达与多种疾病相关。例如,在银屑病中,网络药理学和RNA-seq分析表明,Tpsb2基因可能参与了Th17细胞反应的调控,影响炎症细胞浸润和角质形成细胞异常增殖。此外,代谢组学分析发现,Tpsb2基因的表达可能与氨基酸代谢、肉碱代谢等途径相关[1]。在结直肠癌中,单细胞分析发现Tpsb2基因是肥大细胞的标记基因之一,其表达与肥大细胞的激活状态相关,并且与患者的预后相关[2]。在心房颤动中,Mendelian随机化分析发现Tpsb2基因的表达与心房颤动的风险相关[3]。在骨关节炎中,Tpsb2基因的表达在超重和肥胖患者的滑膜组织中显著升高,可能与滑膜炎症的发生和发展相关[4,9]。在慢性阻塞性肺疾病中,Tpsb2基因的表达与嗜酸性气道炎症和肺功能下降相关[5,6]。在前列腺癌中,Tpsb2基因的表达与患者的预后相关,并且可能参与了 anoikis 相关的生物学过程[8]。

综上所述,Tpsb2基因编码的β-tryptase在多种生物学过程中发挥重要作用,并参与多种疾病的发生和发展。Tpsb2基因的表达受到多种转录因子的调控,并与其他基因和信号通路相互作用,共同影响疾病的病理生理过程。深入研究Tpsb2基因的功能和调控机制,有助于揭示疾病的发病机制,并为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Hu, XueQing, Qi, Cong, Feng, Fang, Li, Ping, Zhao, Jingxia. 2022. Combining network pharmacology, RNA-seq, and metabolomics strategies to reveal the mechanism of Cimicifugae Rhizoma - Smilax glabra Roxb herb pair for the treatment of psoriasis. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 105, 154384. doi:10.1016/j.phymed.2022.154384. https://pubmed.ncbi.nlm.nih.gov/35963195/
2. Xie, Zhenyu, Niu, Liaoran, Zheng, Gaozan, Zhang, Jian, Zheng, Jianyong. 2023. Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment. In Cell & bioscience, 13, 217. doi:10.1186/s13578-023-01144-x. https://pubmed.ncbi.nlm.nih.gov/38031173/
3. Zhang, Yujun, Lian, Qiufang, Nie, Yanwu, Zhao, Wei. 2024. Identification of atrial fibrillation-related genes through transcriptome data analysis and Mendelian randomization. In Frontiers in cardiovascular medicine, 11, 1414974. doi:10.3389/fcvm.2024.1414974. https://pubmed.ncbi.nlm.nih.gov/39055656/
4. Tsuchiya, Maho, Fukushima, Kensuke, Takata, Ken, Takaso, Masashi, Uchida, Kentaro. 2023. Increase in TPSB2 and TPSD1 Expression in Synovium of Hip Osteoarthritis Patients Who Are Overweight. In International journal of molecular sciences, 24, . doi:10.3390/ijms241411532. https://pubmed.ncbi.nlm.nih.gov/37511292/
5. Winter, Natasha A, Gibson, Peter G, McDonald, Vanessa M, Fricker, Michael. 2021. Sputum Gene Expression Reveals Dysregulation of Mast Cells and Basophils in Eosinophilic COPD. In International journal of chronic obstructive pulmonary disease, 16, 2165-2179. doi:10.2147/COPD.S305380. https://pubmed.ncbi.nlm.nih.gov/34321876/
6. Winter, Natasha A, Qin, Ling, Gibson, Peter G, Evans, Tiffany-Jane, Fricker, Michael. 2021. Sputum mast cell/basophil gene expression relates to inflammatory and clinical features of severe asthma. In The Journal of allergy and clinical immunology, 148, 428-438. doi:10.1016/j.jaci.2021.01.033. https://pubmed.ncbi.nlm.nih.gov/33609626/
7. Ohneda, Kinuko, Ohmori, Shin'ya, Yamamoto, Masayuki. 2019. Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. In International journal of molecular sciences, 20, . doi:10.3390/ijms20184603. https://pubmed.ncbi.nlm.nih.gov/31533351/
8. Zhang, Peipei, Lv, Wenzhi, Luan, Yang, Min, Xiangde, Feng, Zhaoyan. . Identification and validation of a novel anoikis-related prognostic model for prostate cancer. In Molecular genetics & genomic medicine, 12, e2419. doi:10.1002/mgg3.2419. https://pubmed.ncbi.nlm.nih.gov/38572916/
9. Takata, Ken, Uchida, Kentaro, Mukai, Manabu, Inoue, Gen, Takaso, Masashi. 2020. Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee. In Diabetes, metabolic syndrome and obesity : targets and therapy, 13, 1491-1497. doi:10.2147/DMSO.S253147. https://pubmed.ncbi.nlm.nih.gov/32440178/
10. Fukuoka, Yoshihiro, Schwartz, Lawrence B. 2007. Active monomers of human beta-tryptase have expanded substrate specificities. In International immunopharmacology, 7, 1900-8. doi:. https://pubmed.ncbi.nlm.nih.gov/18039527/