Atf3-KO 基因敲除小鼠

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产品名称

Atf3-KO 基因敲除小鼠

产品编号

S-KO-01154

品系全称

C57BL/6JCya-Atf3em1/Cya

品系背景

C57BL/6JCya

品系编号

KOCMP-11910-Atf3-B6J-VA

品系状态

使用本品系发表的文献需注明: Atf3-KO 基因敲除小鼠 mice (Strain S-KO-01154) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量

基本信息

基因研究概述

质控标准

基因
基因全称
activating transcription factor 3
基因别称
LRG-21
染色体号
Chr 1 (Mouse)
转录本 ID
NCBI: NM_007498 | Ensembl: ENSMUST00000027941
修饰方式
全身性基因敲除
靶向范围
Exon 3~4
敲除长度
~2.2 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:109384Homozygous null mice display enhanced allergen-induced airway hyperresponsiveness, pulmonary eosinophilia, and chemokine and Th2 cytokine responses in lung tissue and lung-derived CD4+ lymphocytes. Primary pancreatic islets are partially protected from cytokine- or nitric oxide-induced apoptosis.
Atf3,也称为激活转录因子3,是一种在许多生理压力下被诱导的转录因子,属于激活转录因子/环磷酸腺苷反应元件结合蛋白(ATF/CREB)家族。Atf3基因编码的蛋白质参与调节多种生物学过程,包括细胞应激反应、炎症、细胞分化和组织修复等。Atf3基因的表达受到多种信号的调控,如氧化应激、DNA损伤、炎症因子等。Atf3蛋白通过与DNA结合,直接或间接地调节下游基因的表达,从而影响细胞的生物学行为。

Atf3基因在多种疾病的发生和发展中发挥重要作用。例如,在非小细胞肺癌(NSCLC)中,Atf3的表达上调,并且与NSCLC的肿瘤发生有关[9]。此外,在甲状腺癌中,Atf3的表达下调,并且与甲状腺癌的进展和预后相关[2]。Atf3基因的表达还受到DNA甲基化的调控,DNA甲基化可以导致Atf3基因的表达下调,从而影响甲状腺癌的发生和发展[2]。

Atf3基因的表达与多种生物学过程相关。例如,在骨骼肌中,Atf3的表达可以抑制骨骼肌干细胞的过早激活,从而维持骨骼肌组织的稳态和损伤修复[3]。此外,Atf3基因的表达还可以促进皮肤伤口愈合过程中的纤维母细胞活化和胶原产生,从而加速伤口愈合[7]。

Atf3基因的表达还受到其他转录因子的调控。例如,在乳腺癌细胞中,Atf3的表达受到转化生长因子β(TGFβ)的诱导,并且Atf3基因的表达可以增强TGFβ信号通路,从而影响乳腺癌细胞的生物学行为[8]。此外,Atf3基因的表达还受到细胞内ROS水平和DNA双链断裂的影响,从而影响细胞的应激反应和再生能力[4]。

综上所述,Atf3基因是一种重要的转录因子,参与调节多种生物学过程,包括细胞应激反应、炎症、细胞分化和组织修复等。Atf3基因的表达受到多种信号的调控,并且在多种疾病的发生和发展中发挥重要作用。深入研究Atf3基因的功能和调控机制,有助于揭示疾病的发生和发展机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Middleton, Justin D, Fehlman, Jared, Sivakumar, Subhakeertana, Stover, Daniel G, Hai, Tsonwin. 2021. Stress-Inducible Gene Atf3 Dictates a Dichotomous Macrophage Activity in Chemotherapy-Enhanced Lung Colonization. In International journal of molecular sciences, 22, . doi:10.3390/ijms22147356. https://pubmed.ncbi.nlm.nih.gov/34298975/
2. Xiao, Xi, Chen, Mengke, Sang, Ye, Liu, Rengyun, Xiao, Haipeng. 2023. Methylation-Mediated Silencing of ATF3 Promotes Thyroid Cancer Progression by Regulating Prognostic Genes in the MAPK and PI3K/AKT Pathways. In Thyroid : official journal of the American Thyroid Association, 33, 1441-1454. doi:10.1089/thy.2023.0157. https://pubmed.ncbi.nlm.nih.gov/37742107/
3. Zhang, Suyang, Yang, Feng, Huang, Yile, Sun, Hao, Wang, Huating. 2023. ATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expression. In Nature communications, 14, 4978. doi:10.1038/s41467-023-40465-w. https://pubmed.ncbi.nlm.nih.gov/37591871/
4. Zhang, Yingchi, Xu, Tao, Xie, Jie, Hu, Weihua, Yuan, Xuefeng. 2024. MSC-derived mitochondria promote axonal regeneration via Atf3 gene up-regulation by ROS induced DNA double strand breaks at transcription initiation region. In Cell communication and signaling : CCS, 22, 240. doi:10.1186/s12964-024-01617-7. https://pubmed.ncbi.nlm.nih.gov/38664711/
5. Kurniawan, Alfan, Natsir, Muhammad Halim, Suyadi, Suyadi, Furqon, Ahmad, Lestari, Suci Puji. 2023. The effect of feeding with different protein levels on internal organ weight and gene expression of MEF2A and ATF3 in crossbred local chicken using RT-PCR. In Journal, genetic engineering & biotechnology, 21, 83. doi:10.1186/s43141-023-00533-6. https://pubmed.ncbi.nlm.nih.gov/37566282/
6. Liang, G, Wolfgang, C D, Chen, B P, Chen, T H, Hai, T. . ATF3 gene. Genomic organization, promoter, and regulation. In The Journal of biological chemistry, 271, 1695-701. doi:. https://pubmed.ncbi.nlm.nih.gov/8576171/
7. Luo, Peng, Wang, Fulong, Li, Jialun, Wu, Gang, Shi, Chunmeng. 2024. The stress-responsive gene ATF3 drives fibroblast activation and collagen production through transcriptionally activating TGF-β receptor Ⅱ in skin wound healing. In Archives of biochemistry and biophysics, 760, 110134. doi:10.1016/j.abb.2024.110134. https://pubmed.ncbi.nlm.nih.gov/39181381/
8. Yin, Xin, Wolford, Christopher C, Chang, Yi-Seok, Aderem, Alan, Hai, Tsonwin. . ATF3, an adaptive-response gene, enhances TGF{beta} signaling and cancer-initiating cell features in breast cancer cells. In Journal of cell science, 123, 3558-65. doi:10.1242/jcs.064915. https://pubmed.ncbi.nlm.nih.gov/20930144/
9. Song, Xiaoxue, Lu, Fang, Liu, Reng-Yun, Zhou, Qinghua, Zhang, Hong-Tao. . Association between the ATF3 gene and non-small cell lung cancer. In Thoracic cancer, 3, 217-223. doi:10.1111/j.1759-7714.2011.00110.x. https://pubmed.ncbi.nlm.nih.gov/28920309/