Fbxo21-flox 基因敲除小鼠

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产品名称

Fbxo21-flox 基因敲除小鼠

产品编号

S-CKO-17634

品系全称

C57BL/6NCya-Fbxo21em1flox/Cya

品系背景

C57BL/6NCya

品系编号

CKOCMP-231670-Fbxo21-B6N-VB

品系状态

使用本品系发表的文献需注明: Fbxo21-flox 基因敲除小鼠 mice (Strain S-CKO-17634) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
F-box protein 21
基因别称
2810425J22Rik
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_145564 | Ensembl: ENSMUST00000202447
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~0.6 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1924223Mice homozygous for a conditional allele activated in hematopoietic cells exhibit increased susceptibility to 5-FU-induced bone marrow failure and lethality.
FBXO21,也称为F-box only protein 21,是一种重要的F-box蛋白。F-box蛋白是一类参与SCF(Skp1-Cul1-F-box protein)型E3泛素连接酶复合物形成的蛋白质,该复合物在细胞内蛋白的泛素化、降解和调控中发挥着关键作用。FBXO21作为SCF型E3泛素连接酶复合物中的底物识别亚基,通过与底物蛋白的相互作用,介导其泛素化并促进其通过蛋白酶体途径降解。

FBXO21在多种生物学过程中发挥着重要作用,包括细胞分化、发育、代谢和疾病发生。例如,FBXO21介导了EID1(EP300-interacting inhibitor of differentiation 1)的泛素化和降解,从而影响EID1的转录抑制活性[1]。FBXO21还介导了KRT16(角蛋白16)的泛素化和降解,抑制了KRT16驱动的肺癌转移[2]。此外,FBXO21的表达水平与肾移植急性排斥反应相关,FBXO21的表达水平下降与急性排斥反应的发生有关[3]。FBXO21的表达还与脂肪组织的代谢病理生理过程相关,FBXO21的表达水平与脂肪组织的炎症标记物相关[4]。

FBXO21在病毒感染过程中也发挥着重要作用。FBXO21促进Lys29连接的ASK1(apoptosis signal-regulating kinase 1)的泛素化和激活,从而增强病毒感染后的抗病毒天然免疫反应[5]。此外,FBXO21的表达水平与肺癌的发生发展相关,FBXO21的表达水平升高与肺癌的发生发展相关[6]。FBXO21的表达水平还与动脉粥样硬化的发生发展相关,FBXO21的表达水平升高与动脉粥样硬化的发生发展相关[7]。FBXO21的表达水平与卵巢癌的发生发展相关,FBXO21的表达水平升高与卵巢癌的发生发展相关[8]。

FBXO21的表达水平还与高脂饮食小鼠的血脂代谢相关,FBXO21的表达水平升高与血脂代谢异常相关[9]。此外,FBXO21的表达水平与SARS-CoV-2感染后的宿主转录组降解相关,FBXO21的表达水平升高与SARS-CoV-2感染后的宿主转录组降解相关[10]。

综上所述,FBXO21是一种重要的F-box蛋白,在多种生物学过程中发挥着重要作用,包括细胞分化、发育、代谢和疾病发生。FBXO21的表达水平与多种疾病的发生发展相关,包括肺癌、动脉粥样硬化和卵巢癌。此外,FBXO21的表达水平还与高脂饮食小鼠的血脂代谢和SARS-CoV-2感染后的宿主转录组降解相关。FBXO21的研究有助于深入理解F-box蛋白在细胞内蛋白的泛素化、降解和调控中的作用,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Watanabe, Koki, Yumimoto, Kanae, Nakayama, Keiichi I. 2015. FBXO21 mediates the ubiquitylation and proteasomal degradation of EID1. In Genes to cells : devoted to molecular & cellular mechanisms, 20, 667-74. doi:10.1111/gtc.12260. https://pubmed.ncbi.nlm.nih.gov/26085330/
2. Wang, Wen, Zhu, Lifei, Zhou, Jiao, Pei, Xiaofeng, Zhang, Hongyu. 2023. Targeting the KRT16-vimentin axis for metastasis in lung cancer. In Pharmacological research, 193, 106818. doi:10.1016/j.phrs.2023.106818. https://pubmed.ncbi.nlm.nih.gov/37315823/
3. Shaw, Brian I, Cheng, Daniel K, Acharya, Chaitanya R, Kirk, Allan D, Chambers, Eileen T. 2020. An age-independent gene signature for monitoring acute rejection in kidney transplantation. In Theranostics, 10, 6977-6986. doi:10.7150/thno.42110. https://pubmed.ncbi.nlm.nih.gov/32550916/
4. Crujeiras, Ana B, Pissios, Pavlos, Moreno-Navarrete, Jose M, Casanueva, Felipe F, Fernandez-Real, Jose M. 2018. An Epigenetic Signature in Adipose Tissue Is Linked to Nicotinamide N-Methyltransferase Gene Expression. In Molecular nutrition & food research, 62, e1700933. doi:10.1002/mnfr.201700933. https://pubmed.ncbi.nlm.nih.gov/29688621/
5. Yu, Zhou, Chen, Taoyong, Li, Xuelian, Cao, Xuetao, Wang, Jianli. 2016. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. In eLife, 5, . doi:10.7554/eLife.14087. https://pubmed.ncbi.nlm.nih.gov/27063938/
6. Jia, Erna, Ren, Na, Zhang, Rongkui, Zhou, Changyu, Xue, Jinru. . Circulating miR-17 as a promising diagnostic biomarker for lung adenocarcinoma: evidence from the Gene Expression Omnibus. In Translational cancer research, 9, 5544-5554. doi:10.21037/tcr-19-3025. https://pubmed.ncbi.nlm.nih.gov/35117918/
7. Zhang, Yuan-Meng, Meng, Ling-Bing, Yu, Si-Jun, Ma, Dong-Xing. . Identification of potential crucial genes in monocytes for atherosclerosis using bioinformatics analysis. In The Journal of international medical research, 48, 300060520909277. doi:10.1177/0300060520909277. https://pubmed.ncbi.nlm.nih.gov/32314637/
8. Li, Yan, Wang, Juan, Wang, Fang, Cao, Yuanyuan, Wang, Jianhua. 2021. Identification of Specific Cell Subpopulations and Marker Genes in Ovarian Cancer Using Single-Cell RNA Sequencing. In BioMed research international, 2021, 1005793. doi:10.1155/2021/1005793. https://pubmed.ncbi.nlm.nih.gov/34660776/
9. Nan, Guohui, Liu, Lisong, Wu, Huala, Chen, Hui, Wu, Qi. 2022. Transcriptomic and Metabonomic Profiling Reveals the Antihyperlipidemic Effects of Tartary Buckwheat Sprouts in High-Fat-Diet-Fed Mice. In Journal of agricultural and food chemistry, 70, 13302-13312. doi:10.1021/acs.jafc.2c05382. https://pubmed.ncbi.nlm.nih.gov/36215169/
10. Pasquier, Claude, Robichon, Alain. 2021. Computational search of hybrid human/SARS-CoV-2 dsRNA reveals unique viral sequences that diverge from those of other coronavirus strains. In Heliyon, 7, e07284. doi:10.1016/j.heliyon.2021.e07284. https://pubmed.ncbi.nlm.nih.gov/34179538/