Nek6-flox 基因敲除小鼠

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产品名称

Nek6-flox 基因敲除小鼠

产品编号

S-CKO-12520

品系全称

C57BL/6JCya-Nek6em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-59126-Nek6-B6J-VA

品系状态

使用本品系发表的文献需注明: Nek6-flox 基因敲除小鼠 mice (Strain S-CKO-12520) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
NIMA (never in mitosis gene a)-related expressed kinase 6
基因别称
1300007C09Rik
染色体号
Chr 2 (Mouse)
转录本 ID
NCBI: NM_021606.3 | Ensembl: ENSMUST00000054234
修饰方式
条件性基因敲除
靶向范围
Exon 6~7
敲除长度
~1.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1891638No significant homozygous or heterozygous mutant phenotype was detected in a high throughput screen of a targeted mutation, however these homozygotes exhibit an increased response of the heart to induced stress, with aggravated cardiac hypertrophy.
NEK6,全称为“Never in mitosis gene A-related kinase-6”,是一种重要的丝氨酸/苏氨酸激酶,参与调控细胞周期和细胞增殖。NEK6在多种生物学过程中发挥作用,包括细胞分化、发育、代谢和疾病发生。在肿瘤发生发展过程中,NEK6的表达和功能变化可能与肿瘤的恶性行为和预后相关。

根据文献报道,NEK6在多种癌症中表达上调,包括肾癌、前列腺癌、肝细胞癌、卵巢癌和头颈鳞状细胞癌。在肾癌中,NEK6作为风险模型的一部分,与患者的预后不良相关[1]。在前列腺癌中,NEK6调控细胞的氧化还原平衡和DNA损伤反应,其缺失导致细胞死亡,提示NEK6可能是治疗去势抵抗性前列腺癌的新策略[3]。在肝细胞癌中,NEK6的表达上调与肿瘤的分级、α-胎蛋白水平和增殖标记Ki-67相关,且NEK6是HCC的独立预后因素[5]。在卵巢癌中,NEK6通过抑制FOXO3的核转位,稳定C-MYC并促进脱氧嘌呤合成,从而支持卵巢癌的化疗耐药性[6]。在头颈鳞状细胞癌中,NEK6的表达上调与患者预后不良相关,并可能与免疫细胞浸润和免疫治疗的反应相关[9]。

NEK6的功能和作用机制也引起了研究者的关注。在糖尿病心肌病中,NEK6通过调节热休克蛋白72(HSP72)的表达和磷酸化,减轻炎症和氧化应激,保护心脏功能[2]。在巨噬细胞极化中,NEK6的表达下调导致巨噬细胞向促炎表型极化,并通过抑制STAT3的表达来调节巨噬细胞的极化[4]。此外,NEK6还可能通过调节β-catenin/c-Myc通路和STAT3信号通路来影响肿瘤的发生发展[7,8]。

综上所述,NEK6是一种重要的丝氨酸/苏氨酸激酶,参与调控细胞周期、细胞增殖、氧化还原平衡和DNA损伤反应等生物学过程。NEK6在多种癌症中表达上调,并与肿瘤的恶性行为和预后相关。NEK6的功能和作用机制可能涉及调节HSP72、FOXO3、STAT3和β-catenin/c-Myc通路等。NEK6的研究有助于深入理解肿瘤的发生发展机制,为肿瘤的治疗和预防提供新的思路和策略。

参考文献:
1. Li, Song-Chao, Jia, Zhan-Kui, Yang, Jin-Jian, Ning, Xiang-Hui. 2022. Telomere-related gene risk model for prognosis and drug treatment efficiency prediction in kidney cancer. In Frontiers in immunology, 13, 975057. doi:10.3389/fimmu.2022.975057. https://pubmed.ncbi.nlm.nih.gov/36189312/
2. Shao, Shuangyin, Xiao, Lili, Jia, Meng, Wang, Xiaofang, Gao, Lu. 2023. Never in mitosis gene A-related kinase-6 deficiency deteriorates diabetic cardiomyopathy via regulating heat shock protein 72. In Journal of molecular medicine (Berlin, Germany), 101, 419-430. doi:10.1007/s00109-023-02295-7. https://pubmed.ncbi.nlm.nih.gov/36867206/
3. Pavan, Isadora Carolina Betim, Basei, Fernanda Luisa, Severino, Matheus Brandemarte, Simabuco, Fernando Moreira, Kobarg, Jörg. 2023. NEK6 Regulates Redox Balance and DNA Damage Response in DU-145 Prostate Cancer Cells. In Cells, 12, . doi:10.3390/cells12020256. https://pubmed.ncbi.nlm.nih.gov/36672191/
4. Wu, Xiaoyan, Deng, Ke-Qiong, Cai, Huan-Huan, Lu, Zhibing, Cheng, Wen-Lin. 2023. Nek6 knockdown polarized macrophages into a pro-inflammatory phenotype via inhibiting STAT3 expression. In International journal of experimental pathology, 104, 237-246. doi:10.1111/iep.12489. https://pubmed.ncbi.nlm.nih.gov/37431082/
5. Cao, Xiaolei, Xia, Yunfei, Yang, Junling, Li, Liren, Gu, Zhifeng. 2011. Clinical and biological significance of never in mitosis gene A-related kinase 6 (NEK6) expression in hepatic cell cancer. In Pathology oncology research : POR, 18, 201-7. doi:10.1007/s12253-011-9429-0. https://pubmed.ncbi.nlm.nih.gov/21725899/
6. Liu, Jingchun, Wang, Haoyu, Wan, Huanzhi, Yang, Lian, Hong, Li. 2024. NEK6 dampens FOXO3 nuclear translocation to stabilize C-MYC and promotes subsequent de novo purine synthesis to support ovarian cancer chemoresistance. In Cell death & disease, 15, 661. doi:10.1038/s41419-024-07045-2. https://pubmed.ncbi.nlm.nih.gov/39256367/
7. Ting, Gong, Li, Xin, Kwon, Hyog Young, Liu, Youtan, Yang, Yinggui. 2020. microRNA-219-5p targets NEK6 to inhibit hepatocellular carcinoma progression. In American journal of translational research, 12, 7528-7541. doi:. https://pubmed.ncbi.nlm.nih.gov/33312387/
8. Zhu, Min, Sun, Yuyu, Xue, Huawei, Gu, Baorong, Yan, Xiaoling. 2023. NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p. In International journal of general medicine, 16, 2831-2848. doi:10.2147/IJGM.S413461. https://pubmed.ncbi.nlm.nih.gov/37426517/
9. Yang, Zhi-Min, Liao, Bing, Yang, Si-Si, Zhang, Jing, Wang, Wei-Ming. 2022. Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma. In Frontiers in endocrinology, 13, 943686. doi:10.3389/fendo.2022.943686. https://pubmed.ncbi.nlm.nih.gov/35898455/