Nell2-flox 基因敲除小鼠

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产品名称

Nell2-flox 基因敲除小鼠

产品编号

S-CKO-11673

品系全称

C57BL/6JCya-Nell2em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-54003-Nell2-B6J-VA

品系状态

使用本品系发表的文献需注明: Nell2-flox 基因敲除小鼠 mice (Strain S-CKO-11673) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
NEL-like 2
基因别称
A330108N19Rik,mel91
染色体号
Chr 15 (Mouse)
转录本 ID
NCBI: NM_001356950 | Ensembl: ENSMUST00000166170
修饰方式
条件性基因敲除
靶向范围
Exon 4
敲除长度
~0.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1858510Homozygous null mice display enhanced long term potentiation in the dentate gyrus of the hippocampus. Male mice homozygous for a null allele exhibit infertility, impaired binding of sperm to zona pellucida, and impaired sperm migration in female genital tract.
NELL2,也称为神经组织特异性表皮生长因子样重复域蛋白,是一种在神经组织中主要表达的分泌性糖蛋白。NELL2含有六个表皮生长因子样结构域,这些结构域在蛋白质的功能中起着重要作用。NELL2在神经发育、细胞存活和神经元功能等方面发挥着关键作用,其表达和活性受到多种因素的调控,包括雌激素、细胞内信号通路和细胞外信号。

NELL2在多种生物学过程中发挥重要作用,包括细胞存活、神经发育和肿瘤发生。在细胞存活方面,NELL2能够保护细胞免受多种细胞死亡诱导环境的影响,如内质网应激和氧化应激。NELL2还能够促进神经元的存活和分化,这对于神经系统的发育和功能至关重要。在肿瘤发生方面,NELL2的表达和活性与多种肿瘤的发生和发展密切相关,包括肺癌、乳腺癌和结直肠癌等。

NELL2的表达和活性受到多种因素的调控,包括雌激素、细胞内信号通路和细胞外信号。雌激素能够刺激NELL2的转录,从而保护细胞免受细胞死亡诱导环境的影响。细胞内信号通路,如ERK信号通路,也能够调节NELL2的表达和活性,从而影响细胞存活和神经元功能。细胞外信号,如NELL2-ROS1信号通路,也能够调节NELL2的表达和活性,从而影响精子成熟和肿瘤发生。

NELL2是一种在神经组织中主要表达的分泌性糖蛋白,其含有六个表皮生长因子样结构域。NELL2在神经发育、细胞存活和神经元功能等方面发挥着关键作用,其表达和活性受到多种因素的调控,包括雌激素、细胞内信号通路和细胞外信号。NELL2的研究有助于深入理解神经系统的发育和功能,以及肿瘤发生和发展的机制,为相关疾病的治疗和预防提供新的思路和策略[1][2][3][4][5][6][7][8][9][10]。

参考文献:
1. Ha, Chang Man, Kim, Dong Hee, Lee, Tae Hwan, Jeong, Jin Kwon, Lee, Byung Ju. 2022. Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression. In Molecules and cells, 45, 537-549. doi:10.14348/molcells.2022.2051. https://pubmed.ncbi.nlm.nih.gov/35950455/
2. Zhang, Zhenxing, Zhu, Haoran, Wang, Xiaojun, Ruan, Chenjin, Wang, Qiang. 2023. A novel basement membrane-related gene signature for prognosis of lung adenocarcinomas. In Computers in biology and medicine, 154, 106597. doi:10.1016/j.compbiomed.2023.106597. https://pubmed.ncbi.nlm.nih.gov/36708655/
3. Munemasa, Yasunari, Chang, Chang-Sheng, Kwong, Jacky M K, Caprioli, Joseph, Piri, Natik. 2012. The neuronal EGF-related gene Nell2 interacts with Macf1 and supports survival of retinal ganglion cells after optic nerve injury. In PloS one, 7, e34810. doi:10.1371/journal.pone.0034810. https://pubmed.ncbi.nlm.nih.gov/22496866/
4. Jayabal, Panneerselvam, Zhou, Fuchun, Lei, Xiufen, Houghton, Peter J, Shiio, Yuzuru. . NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth. In Cell reports, 36, 109254. doi:10.1016/j.celrep.2021.109254. https://pubmed.ncbi.nlm.nih.gov/34233189/
5. Jeong, Jin Kwon, Kim, Jae Geun, Kim, Han Rae, Park, Jeong Woo, Lee, Byung Ju. 2017. A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats. In Molecules and cells, 40, 186-194. doi:10.14348/molcells.2017.2278. https://pubmed.ncbi.nlm.nih.gov/28301916/
6. Choi, Eun Jung, Kim, Dong Hee, Kim, Jae Geun, Ojeda, Sergio R, Lee, Byung Ju. 2010. Estrogen-dependent transcription of the NEL-like 2 (NELL2) gene and its role in protection from cell death. In The Journal of biological chemistry, 285, 25074-84. doi:10.1074/jbc.M110.100545. https://pubmed.ncbi.nlm.nih.gov/20538601/
7. Kim, Dong Yeol, Kim, Han Rae, Kim, Kwang Kon, Park, Jeong Woo, Lee, Byung Ju. 2014. NELL2 function in the protection of cells against endoplasmic reticulum stress. In Molecules and cells, 38, 145-50. doi:10.14348/molcells.2015.2216. https://pubmed.ncbi.nlm.nih.gov/25537860/
8. Kim, Dong Hee, Roh, Yun-Gil, Lee, Hyun Hee, Lee, Byung Ju, Leem, Sun-Hee. 2013. The E2F1 oncogene transcriptionally regulates NELL2 in cancer cells. In DNA and cell biology, 32, 517-23. doi:10.1089/dna.2013.1974. https://pubmed.ncbi.nlm.nih.gov/23829315/
9. Kiyozumi, Daiji. . The molecular mechanisms of mammalian sperm maturation regulated by NELL2-ROS1 lumicrine signaling. In Journal of biochemistry, 172, 341-346. doi:10.1093/jb/mvac071. https://pubmed.ncbi.nlm.nih.gov/36071564/
10. Kim, Dong-Gyu, Hwang, Eun Mi, Yoo, Jae Cheal, Hong, Seong-Geun, Park, Jae-Yong. 2009. Identification and characterization of a truncated isoform of NELL2. In Biochemical and biophysical research communications, 391, 529-34. doi:10.1016/j.bbrc.2009.11.092. https://pubmed.ncbi.nlm.nih.gov/19931511/