Rfx5-flox 基因敲除小鼠

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产品名称

Rfx5-flox 基因敲除小鼠

产品编号

S-CKO-11669

品系全称

C57BL/6JCya-Rfx5em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-53970-Rfx5-B6J-VA

品系状态

使用本品系发表的文献需注明: Rfx5-flox 基因敲除小鼠 mice (Strain S-CKO-11669) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
regulatory factor X, 5 (influences HLA class II expression)
基因别称
-
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_017395.3 | Ensembl: ENSMUST00000137088
修饰方式
条件性基因敲除
靶向范围
Exon 5~8
敲除长度
~1.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1858421Homozygous null mice have absent or decreased expression of MHC-II complexes on antigen presenting cells, which leads to reduced numbers of CD4+ thymocytes and T cells.
RFX5,也称为regulatory factor X 5,是翼螺旋家族(winged HLH family)的成员。RFX5蛋白含有四个结构域:寡聚化结构域、DNA结合域、螺旋结构域和激活域。RFX5在免疫系统中起着关键作用,是主要组织相容性复合物II类(MHC II)基因表达的重要调节因子[6]。RFX5参与多种生物学过程,包括免疫反应、细胞周期调控和细胞凋亡等[1,2,3,4,5,6]。

在免疫系统中,RFX5主要参与MHC II基因的表达调控。MHC II基因复合物是免疫系统中的重要组成部分,负责抗原呈递和免疫识别。RFX5通过与MHC II基因启动子区域的DNA结合,激活MHC II基因的表达,从而参与免疫反应的调控[6]。此外,RFX5还可以与其他转录因子形成复合物,进一步影响免疫相关基因的表达[9]。

除了在免疫系统中发挥作用,RFX5还在其他生物学过程中发挥作用。例如,RFX5可以调控原钙粘蛋白(Pcdh)基因簇的表达。Pcdh基因簇在大脑发育中起着关键作用,参与神经元自避免的形成。研究表明,RFX5的缺失会导致Pcdhα基因簇的表达水平显著升高[1]。此外,RFX5还可以调控细胞周期和细胞凋亡相关基因的表达,从而影响细胞生长和细胞死亡[2,3,4,5]。

RFX5的功能还受到其他蛋白质的调节。例如,SIRT1可以与RFX5形成复合物,并通过去乙酰化作用抑制RFX5的活性,从而影响胶原蛋白的转录[10]。此外,RFX5的表达水平还与肿瘤的预后和免疫浸润程度相关[7,8]。

综上所述,RFX5是一种重要的转录因子,参与免疫反应、细胞周期调控和细胞凋亡等多种生物学过程。RFX5的功能受到其他蛋白质的调节,并且与肿瘤的预后和免疫浸润程度相关。深入研究RFX5的功能和调节机制,有助于理解免疫系统的调控机制和肿瘤的发生发展过程,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Wang, Na, Jia, Zhi Lian, Wu, Qiang. . RFX5 regulates gene expression of the Pcdhα cluster. In Yi chuan = Hereditas, 42, 760-774. doi:10.16288/j.yczz.20-184. https://pubmed.ncbi.nlm.nih.gov/32952112/
2. Zhang, Jun-Jie, Shen, Yan, Chen, Xiao-Yuan, Zhang, Jie, Xu, Fei. 2023. Integrative network-based analysis on multiple Gene Expression Omnibus datasets identifies novel immune molecular markers implicated in non-alcoholic steatohepatitis. In Frontiers in endocrinology, 14, 1115890. doi:10.3389/fendo.2023.1115890. https://pubmed.ncbi.nlm.nih.gov/37008925/
3. Zhao, Yangjing, Xie, Xingwang, Liao, Weijia, Shao, Qixiang, Chen, Hongsong. 2016. The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma. In Oncology reports, 37, 289-296. doi:10.3892/or.2016.5240. https://pubmed.ncbi.nlm.nih.gov/27840983/
4. Agrafioti, Panagiota, Morin-Baxter, Joshua, Tanagala, Kranthi K K, Lalla, Evanthia, Momen-Heravi, Fatemeh. . Decoding the role of macrophages in periodontitis and type 2 diabetes using single-cell RNA-sequencing. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22136. doi:10.1096/fj.202101198R. https://pubmed.ncbi.nlm.nih.gov/35032412/
5. Chen, Dong-Bo, Xie, Xing-Wang, Zhao, Yang-Jing, Wei, Lai, Chen, Hong-Song. 2020. RFX5 promotes the progression of hepatocellular carcinoma through transcriptional activation of KDM4A. In Scientific reports, 10, 14538. doi:10.1038/s41598-020-71403-1. https://pubmed.ncbi.nlm.nih.gov/32883983/
6. Laird, Kholiswa M, Briggs, LaTese L, Boss, Jeremy M, Summers, Michael F, Garvie, Colin W. 2010. Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex. In Journal of molecular biology, 403, 40-51. doi:10.1016/j.jmb.2010.08.025. https://pubmed.ncbi.nlm.nih.gov/20732328/
7. Wang, Haoyu, Liu, Jingchun, Yang, Jiang, Wang, Ying, Hong, Li. 2022. A novel tumor mutational burden-based risk model predicts prognosis and correlates with immune infiltration in ovarian cancer. In Frontiers in immunology, 13, 943389. doi:10.3389/fimmu.2022.943389. https://pubmed.ncbi.nlm.nih.gov/36003381/
8. Guo, Lili, Liu, Dingsheng. 2022. Identification of RFX5 as prognostic biomarker and associated with immune infiltration in stomach adenocarcinoma. In European journal of medical research, 27, 164. doi:10.1186/s40001-022-00794-w. https://pubmed.ncbi.nlm.nih.gov/36045400/
9. Pelissier, Aurelien, Laragione, Teresina, Gulko, Percio S, Rodríguez Martínez, María. 2024. Cell-specific gene networks and drivers in rheumatoid arthritis synovial tissues. In Frontiers in immunology, 15, 1428773. doi:10.3389/fimmu.2024.1428773. https://pubmed.ncbi.nlm.nih.gov/39161769/
10. Xia, Jun, Wu, Xiaoyan, Yang, Yuyu, Wang, Hong, Xu, Yong. 2012. SIRT1 deacetylates RFX5 and antagonizes repression of collagen type I (COL1A2) transcription in smooth muscle cells. In Biochemical and biophysical research communications, 428, 264-70. doi:10.1016/j.bbrc.2012.10.043. https://pubmed.ncbi.nlm.nih.gov/23079621/