Shisa3-flox 基因敲除小鼠

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产品名称

Shisa3-flox 基因敲除小鼠

产品编号

S-CKO-10659

品系全称

C57BL/6JCya-Shisa3em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-330096-Shisa3-B6J-VA

品系状态

使用本品系发表的文献需注明: Shisa3-flox 基因敲除小鼠 mice (Strain S-CKO-10659) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
shisa family member 3
基因别称
D830007B15Rik
染色体号
Chr 5 (Mouse)
转录本 ID
NCBI: NM_001033415.3 | Ensembl: ENSMUST00000087241
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~710 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:3041225Mice homozygous for a targeted null mutation are viable, fertile and apparently normal with no defects in head development.
Shisa3基因是一种在多种癌症中发挥重要作用的肿瘤抑制基因。Shisa3基因编码的蛋白主要功能是抑制Wnt/β-catenin信号通路,通过降解β-catenin蛋白来抑制肿瘤的发生和发展[1]。Shisa3基因的表达下调或失活,会导致Wnt/β-catenin信号通路的异常激活,从而促进肿瘤细胞的增殖、迁移和侵袭[2]。

Shisa3基因在多种癌症中发挥肿瘤抑制功能,包括三阴性乳腺癌(TNBC)、结直肠癌(CRC)、鼻咽癌(NPC)、慢性淋巴细胞白血病(CLL)和喉鳞状细胞癌(LSCC)等。在三阴性乳腺癌中,Shisa3基因的表达下调与肿瘤的侵袭、转移和不良预后相关[3]。在结直肠癌中,Shisa3基因的启动子区甲基化是肿瘤发生的早期事件,与肿瘤的复发和不良预后相关[4]。在鼻咽癌中,Shisa3基因的表达下调与肿瘤的侵袭和转移相关,其机制可能是通过降低SGSM1的稳定性来实现的[5]。在慢性淋巴细胞白血病中,Shisa3基因的表达上调与患者对lenalidomide的敏感性相关,可以作为预测治疗反应的生物标志物[6]。在喉鳞状细胞癌中,Shisa3基因的启动子区甲基化是肿瘤发生的早期事件,与肿瘤的发生和不良预后相关[7]。

此外,Shisa3基因的表达下调还与肺腺癌患者的总生存期和无进展生存期相关[8]。肺腺癌患者中Shisa3基因的表达下调可能与肿瘤的发生和发展相关,其机制可能是通过促进β-catenin降解来实现的[9]。在Hu羊中,Shisa3基因的SNPs与饲料转化率相关,可以作为提高饲料转化效率的遗传标记[10]。

综上所述,Shisa3基因在多种癌症中发挥肿瘤抑制功能,其表达下调或失活会导致Wnt/β-catenin信号通路的异常激活,从而促进肿瘤的发生和发展。Shisa3基因的表达下调或甲基化可能是肿瘤发生的早期事件,与肿瘤的复发和不良预后相关。Shisa3基因可以作为肿瘤诊断、预后和治疗的生物标志物,其研究有助于深入理解肿瘤的发生和发展机制,为肿瘤的治疗和预防提供新的思路和策略[1-10]。

参考文献:
1. Khaled, Noura, Sonnier, Nicolas, Molnar, Ioana, Privat, Maud, Bidet, Yannick. 2021. RNA sequencing reveals the differential expression profiles of RNA in metastatic triple negative breast cancer and identifies SHISA3 as an efficient tumor suppressor gene. In American journal of cancer research, 11, 4568-4581. doi:. https://pubmed.ncbi.nlm.nih.gov/34659906/
2. Murakami, Kohei, Zhifeng, He, Suzuki, Takako, Kobayashi, Yasuhiro, Nakamura, Yukio. 2019. The Shisa3 knockout mouse exhibits normal bone phenotype. In Journal of bone and mineral metabolism, 37, 967-975. doi:10.1007/s00774-019-01014-y. https://pubmed.ncbi.nlm.nih.gov/31222549/
3. Tang, Sheng-Hui, Hsiao, Cheng-Wen, Chen, Wei-Liang, Chang, Jin-Biou, Yang, Bing-Heng. . Hypermethylation of SHISA3 DNA as a blood-based biomarker for colorectal cancer. In The Chinese journal of physiology, 64, 51-56. doi:10.4103/CJP.CJP_89_20. https://pubmed.ncbi.nlm.nih.gov/33642344/
4. Zhang, Jian, Li, Ying-Qin, Guo, Rui, Ma, Jun, Liu, Na. 2018. Hypermethylation of SHISA3 Promotes Nasopharyngeal Carcinoma Metastasis by Reducing SGSM1 Stability. In Cancer research, 79, 747-759. doi:10.1158/0008-5472.CAN-18-1754. https://pubmed.ncbi.nlm.nih.gov/30573520/
5. Maffei, Rossana, Fiorcari, Stefania, Martinelli, Silvia, Luppi, Mario, Marasca, Roberto. 2017. Increased SHISA3 expression characterizes chronic lymphocytic leukemia patients sensitive to lenalidomide. In Leukemia & lymphoma, 59, 423-433. doi:10.1080/10428194.2017.1339872. https://pubmed.ncbi.nlm.nih.gov/28639485/
6. Tsai, Ming-Hong, Chen, Wen-Chi, Yu, Sung-Liang, Yen, Sou-Jhy, Yang, Ya-Chien. 2015. DNA Hypermethylation of SHISA3 in Colorectal Cancer: An Independent Predictor of Poor Prognosis. In Annals of surgical oncology, 22 Suppl 3, S1481-9. doi:10.1245/s10434-015-4593-1. https://pubmed.ncbi.nlm.nih.gov/25968618/
7. Shahzad, Naveed, Munir, Tehreem, Javed, Mariam, Anjum, Rana Salman, Shakoori, Abdul Rauf. 2020. SHISA3, an antagonist of the Wnt/β-catenin signaling, is epigenetically silenced and its ectopic expression suppresses growth in breast cancer. In PloS one, 15, e0236192. doi:10.1371/journal.pone.0236192. https://pubmed.ncbi.nlm.nih.gov/32692756/
8. Shen, Zhisen, Zhou, Chongchang, Li, Jinyun, Lin, Lexi, Zhang, Yuna. 2017. SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Carcinoma. In BioMed research international, 2017, 9058749. doi:10.1155/2017/9058749. https://pubmed.ncbi.nlm.nih.gov/28299336/
9. Chen, Chun-Chieh, Chen, Hsuan-Yu, Su, Kang-Yi, Yang, Ya-Chien, Yu, Sung-Liang. . Shisa3 is associated with prolonged survival through promoting β-catenin degradation in lung cancer. In American journal of respiratory and critical care medicine, 190, 433-44. doi:10.1164/rccm.201312-2256OC. https://pubmed.ncbi.nlm.nih.gov/25036006/
10. Lin, Changchun, Wang, Weimin, Zhang, Deyin, Zhang, Xiaoxue, Zheng, Wenxin. 2023. Polymorphisms in SHISA3 and RFC3 genes and their association with feed conversion ratio in Hu sheep. In Frontiers in veterinary science, 9, 1010045. doi:10.3389/fvets.2022.1010045. https://pubmed.ncbi.nlm.nih.gov/36686193/