Arhgap44-flox 基因敲除小鼠

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产品名称

Arhgap44-flox 基因敲除小鼠

产品编号

S-CKO-06053

品系全称

C57BL/6JCya-Arhgap44em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-216831-Arhgap44-B6J-VA

品系状态

使用本品系发表的文献需注明: Arhgap44-flox 基因敲除小鼠 mice (Strain S-CKO-06053) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
Rho GTPase activating protein 44
基因别称
6330543G20,Rich2
染色体号
Chr 11 (Mouse)
转录本 ID
NCBI: NM_175003.3 | Ensembl: ENSMUST00000047463
修饰方式
条件性基因敲除
靶向范围
Exon 4
敲除长度
~577 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:2144423Mice homozygous for a knock-out allele exhibit increased brain weight, abnormal dendritic spine morphology, hypoactivity, increased self-grooming behavior, abnormal response to novel objects, and impaired motor learning.
Arhgap44,也称为Rho GTPase-activating protein 44,是一种属于Rho GTPase-activating protein家族的基因。Rho GTPase-activating proteins是一类重要的信号传导分子,参与调控细胞骨架重塑、细胞迁移、细胞粘附和细胞增殖等过程。Arhgap44具有GAP(GTPase-activating protein)活性,可以催化GTP在Rho家族的小G蛋白上的水解,从而调控Rho家族小G蛋白的活性,进而影响细胞的行为和功能。

Arhgap44在多种疾病中发挥重要作用,包括糖尿病、骨肉瘤和白血病。研究发现,Arhgap44的表达与糖化血红蛋白(HbA1c)的水平相关。HbA1c是衡量血糖水平的指标,其水平与胰岛素抵抗和2型糖尿病(T2D)的发生发展密切相关。在非糖尿病人群中,Arhgap44的表达与HbA1c的纵向变化相关,这表明Arhgap44可能参与调节血糖稳态和糖尿病的发生发展[1,2,3]。

在骨肉瘤中,Arhgap44的表达与肿瘤的恶性行为和预后相关。研究发现,Arhgap44在骨肉瘤组织中高表达,与肿瘤的转移和不良预后相关。此外,下调Arhgap44的表达可以抑制骨肉瘤细胞的恶性行为,这表明Arhgap44可能成为骨肉瘤治疗的潜在靶点[4,5]。

在白血病中,Arhgap44的表达与肿瘤的发生发展相关。研究发现,Arhgap44在白血病细胞中表达,其表达水平与肿瘤的发生发展相关。此外,Arhgap44的表达与一些重要的信号通路相关,如Hedgehog信号通路和甾醇生物合成通路,这表明Arhgap44可能通过调控这些信号通路影响白血病的发生发展[6]。

此外,Arhgap44还参与调节神经元的生长和发育。研究发现,Arhgap44在神经元中表达,其表达水平与神经元的生长和发育相关。Arhgap44可以抑制神经元突起的形成,这表明Arhgap44可能参与调节神经元的形态发生和突触的形成[7]。

综上所述,Arhgap44是一种重要的Rho GTPase-activating protein,参与调控细胞骨架重塑、细胞迁移、细胞粘附和细胞增殖等过程。Arhgap44在多种疾病中发挥重要作用,包括糖尿病、骨肉瘤和白血病。Arhgap44的研究有助于深入理解细胞信号传导和疾病发生发展的机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Wang, Siyu, Lenzini, Petra, Thygarajan, Bharat, Province, Michael A, An, Ping. 2024. A Novel Gene ARHGAP44 for Longitudinal Changes in Glycated Hemoglobin (HbA1c) in Subjects without Type 2 Diabetes: Evidence from the Long Life Family Study (LLFS) and the Framingham Offspring Study (FOS). In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.05.16.594575. https://pubmed.ncbi.nlm.nih.gov/38826208/
2. Li, Shizhe, Xue, Jiancheng, Zhang, He, Shang, Guanning. 2023. ARHGAP44-mediated regulation of the p53/C-myc/Cyclin D1 pathway in modulating the malignant biological behavior of osteosarcoma cells. In Journal of orthopaedic surgery and research, 18, 910. doi:10.1186/s13018-023-04406-z. https://pubmed.ncbi.nlm.nih.gov/38031136/
3. Wang, Siyu, Lenzini, Petra, Thyagarajan, Bharat, Province, Michael A, An, Ping. 2025. Evidence of a novel gene locus ARHGAP44 for longitudinal change in hemoglobin A1c levels among subjects without diabetes from the Long Life Family Study. In Physiological genomics, 57, 293-298. doi:10.1152/physiolgenomics.00137.2024. https://pubmed.ncbi.nlm.nih.gov/40019798/
4. Hu, Shouchao, Wang, Wenzhi. 2022. ARHGAP44 expression is associated with the metastasis of osteosarcoma and is a promising prognostic biomarker. In Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 41, 1348-1355. doi:10.1002/jor.25478. https://pubmed.ncbi.nlm.nih.gov/36317850/
5. Xu, Jinjin, Jiao, Jian, Xu, Wei, Xiao, Jianru, Zhang, Bianhong. 2017. Mutant p53 promotes cell spreading and migration via ARHGAP44. In Science China. Life sciences, 60, 1019-1029. doi:10.1007/s11427-016-9040-8. https://pubmed.ncbi.nlm.nih.gov/28527113/
6. Galic, Milos, Tsai, Feng-Chiao, Collins, Sean R, Bandara, Samuel, Meyer, Tobias. 2014. Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. In eLife, 3, e03116. doi:10.7554/eLife.03116. https://pubmed.ncbi.nlm.nih.gov/25498153/
7. Luo, Mei, Miao, Ya-Ru, Ke, Ya-Juan, Guo, An-Yuan, Zhang, Qiong. . A comprehensive landscape of transcription profiles and data resources for human leukemia. In Blood advances, 7, 3435-3449. doi:10.1182/bloodadvances.2022008410. https://pubmed.ncbi.nlm.nih.gov/36595475/