Per3-flox 基因敲除小鼠

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产品名称

Per3-flox 基因敲除小鼠

产品编号

S-CKO-04242

品系全称

C57BL/6JCya-Per3em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-18628-Per3-B6J-VA

品系状态

使用本品系发表的文献需注明: Per3-flox 基因敲除小鼠 mice (Strain S-CKO-04242) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
period circadian clock 3
基因别称
2810049O06Rik,mPer3
染色体号
Chr 4 (Mouse)
转录本 ID
NCBI: NM_011067 | Ensembl: ENSMUST00000103204
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~0.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1277134Mice homozygous for a targeted null mutation exhibit a shorter circadian cycle length.
Per3基因,全称为Period circadian regulator 3,是哺乳动物生物钟系统的重要组成部分。Per3基因编码的蛋白质参与调节昼夜节律,对生物体的生理和行为活动有着深远的影响。Per3基因的表达受到生物钟系统中的其他基因如Per1、Per2和Cry1的调控,形成一个复杂的反馈环路,共同维持生物体的昼夜节律[1]。

Per3基因的表达受到昼夜节律的调节,其表达水平在夜间较高,而在白天较低。这种昼夜节律性的表达模式与生物体的睡眠-觉醒周期相一致,表明Per3基因在调节睡眠-觉醒节律中起着重要作用。Per3基因的表达还受到光照的调节,光照可以通过视网膜传入信号,影响Per3基因的表达,进而调节生物体的昼夜节律[1]。

Per3基因的多态性与多种生理和行为特征有关。例如,Per3基因的长度多态性(VNTR)与睡眠-觉醒节律、睡眠相位延迟综合症和极端昼夜偏好有关[1]。Per3基因的VNTR多态性还可以影响个体对咖啡因的敏感性,进而影响个体的睡眠质量[2]。此外,Per3基因的表达还与肠道微生物群的昼夜节律性变化有关[3]。

Per3基因的突变或缺失会导致昼夜节律紊乱,进而引发一系列生理和行为问题。例如,Per3基因的缺失会导致小鼠的昼夜节律紊乱,表现为睡眠-觉醒周期缩短、睡眠质量下降和活动节律异常[4]。此外,Per3基因的表达还与多种疾病的发生和发展有关,如胶质瘤、前列腺癌和糖尿病神经病变等[2,5,10]。

Per3基因的表达还受到表观遗传调控的影响,如DNA甲基化。Per3基因的DNA甲基化水平可以影响其表达水平,进而影响个体的睡眠质量和心理健康[5]。此外,Per3基因的DNA甲基化水平还可以调节个体对咖啡因的敏感性[6]。

综上所述,Per3基因是哺乳动物生物钟系统的重要组成部分,参与调节昼夜节律、睡眠-觉醒节律和肠道微生物群的昼夜节律性变化。Per3基因的多态性和表达水平与多种生理和行为特征有关,如睡眠-觉醒节律、睡眠质量、肠道微生物群和疾病易感性等。Per3基因的研究有助于深入理解昼夜节律的调控机制和疾病的发生发展机制,为疾病的治疗和预防提供新的思路和策略[7,8,9]。

参考文献:
1. Archer, Simon N, Robilliard, Donna L, Skene, Debra J, Arendt, Josephine, von Schantz, Malcolm. . A length polymorphism in the circadian clock gene Per3 is linked to delayed sleep phase syndrome and extreme diurnal preference. In Sleep, 26, 413-5. doi:. https://pubmed.ncbi.nlm.nih.gov/12841365/
2. Wang, Qingqing, Liu, Huaifeng, Wang, Zhiheng, Gao, Yu, Li, Shujing. 2023. Circadian gene Per3 promotes astroblastoma progression through the P53/BCL2/BAX signalling pathway. In Gene, 895, 147978. doi:10.1016/j.gene.2023.147978. https://pubmed.ncbi.nlm.nih.gov/37951372/
3. Xie, Xiaoxian, Xu, Haoshen, Shu, Ruonan, Li, Zezhi, Wu, Fengchun. . Clock gene Per3 deficiency disrupts circadian alterations of gut microbiota in mice. In Acta biochimica et biophysica Sinica, 55, 2004-2007. doi:10.3724/abbs.2023257. https://pubmed.ncbi.nlm.nih.gov/37964605/
4. Noda, Mariko, Iwamoto, Ikuko, Tabata, Hidenori, Ito, Hidenori, Nagata, Koh-Ichi. 2019. Role of Per3, a circadian clock gene, in embryonic development of mouse cerebral cortex. In Scientific reports, 9, 5874. doi:10.1038/s41598-019-42390-9. https://pubmed.ncbi.nlm.nih.gov/30971765/
5. Li, Tingting, Xie, Yang, Tao, Shuman, Tao, Fangbiao, Wu, Xiaoyan. 2022. Moderating effects of PER3 gene DNA methylation on the association of sleep quality with mental health in Chinese young adults. In Journal of affective disorders, 323, 716-722. doi:10.1016/j.jad.2022.12.048. https://pubmed.ncbi.nlm.nih.gov/36528137/
6. Viana Mendes, Juliana, Benedito-Silva, Ana Amélia, Medeiros Andrade, Maria Augusta, Cipolla-Neto, José, Pedrazzoli, Mario. 2023. Actigraphic characterization of sleep and circadian phenotypes of PER3 gene VNTR genotypes. In Chronobiology international, 40, 1244-1250. doi:10.1080/07420528.2023.2256858. https://pubmed.ncbi.nlm.nih.gov/37691400/
7. Li, Qilin, Xia, Ding, Wang, Zhihua, Li, Qiuhui, Chen, Xin. 2021. Circadian Rhythm Gene PER3 Negatively Regulates Stemness of Prostate Cancer Stem Cells via WNT/β-Catenin Signaling in Tumor Microenvironment. In Frontiers in cell and developmental biology, 9, 656981. doi:10.3389/fcell.2021.656981. https://pubmed.ncbi.nlm.nih.gov/33816508/
8. Nacar, Mehmet Can, Nursal, Ayse Feyda, Kuruca, Nilufer, Yigit, Serbulent. 2022. A circadian rhythm gene (PER3) VNTR variant as possible risk factor in cohort of Turkish females with primary dysmenorrhea. In Nucleosides, nucleotides & nucleic acids, 41, 900-909. doi:10.1080/15257770.2022.2085743. https://pubmed.ncbi.nlm.nih.gov/35707903/
9. Azevedo, Pedro Guimarães de, Miranda, Luana Reis, Nicolau, Eduardo Souza, Marco, Luiz De, Bastos-Rodrigues, Luciana. 2021. Genetic association of the PERIOD3 (PER3) Clock gene with extreme obesity. In Obesity research & clinical practice, 15, 334-338. doi:10.1016/j.orcp.2021.06.006. https://pubmed.ncbi.nlm.nih.gov/34215556/
10. Rüstemoğlu, Hüsniye, Aci, Recai, Uzun, Süheyla, Yiğit, Serbülent, Rüstemoğlu, Aydın. 2023. Investigation of circadian rhythm gene Per3 in diabetic neuropathy. In Nucleosides, nucleotides & nucleic acids, 43, 596-606. doi:10.1080/15257770.2023.2286996. https://pubmed.ncbi.nlm.nih.gov/38037954/