Msx2-flox 基因敲除小鼠

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产品名称

Msx2-flox 基因敲除小鼠

产品编号

S-CKO-03804

品系全称

C57BL/6JCya-Msx2em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-17702-Msx2-B6J-VA

品系状态

使用本品系发表的文献需注明: Msx2-flox 基因敲除小鼠 mice (Strain S-CKO-03804) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
msh homeobox 2
基因别称
Hox-8,Hox8,Hox8.1
染色体号
Chr 13 (Mouse)
转录本 ID
NCBI: NM_013601 | Ensembl: ENSMUST00000021922
修饰方式
条件性基因敲除
靶向范围
Exon 2
敲除长度
~2.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:97169Homozygotes for a targeted null mutation exhibit defective skull ossification with persistent calvarial foramen, alopecia, stubby and curly whiskers, seizures, and impaired development of teeth, cerebellum, and mammary gland.
Msx2,也称为Msh Homeobox 2,是一种重要的同源框基因,在多种生物过程中发挥着关键作用。它编码的蛋白包含一个同源结构域,可以识别并结合特定的DNA序列,从而调控基因表达。Msx2在胚胎发育过程中广泛表达,尤其在眼睛、牙齿、骨骼和皮肤等组织和器官中发挥重要作用。Msx2基因的表达模式复杂,受多种因素的调控,包括YY1蛋白、骨形态发生蛋白(BMPs)等[3]。

Msx2基因在多种生物过程中发挥着关键作用。例如,Msx2可以抑制鹅真皮成纤维细胞的凋亡并促进其增殖,从而影响鹅绒毛的生长发育[1]。在牦牛的颗粒细胞中,Msx2的表达水平与细胞活力和甾体生成密切相关[2]。此外,Msx2基因的拷贝数增加还与某些眼部发育缺陷相关[4]。Msx2还可以调控牙齿发育过程中釉蛋白的表达,影响牙齿的形态和质量[5][7]。在人类胎盘发育过程中,Msx2可以维持滋养层干细胞的分化命运,防止其过早分化为合体滋养层细胞[6]。此外,Msx2基因的表达还受到甾体激素的调控,在乳腺癌组织中发挥重要作用[10]。

Msx1和Msx2基因的表达在镉诱导的鸡胚胎脐膨出模型中被下调,提示Msx基因可能在胚胎发育过程中发挥重要作用,并通过调控细胞增殖和分化影响胚胎发育[8]。Msx2和骨钙素基因在鼠牙发生过程中的表达呈现相互拮抗的模式,提示Msx2可能在牙齿发育过程中发挥负调控作用[9]。

Msx2基因的研究为深入理解其在多种生物过程中的作用机制提供了重要线索。未来研究可以进一步探索Msx2基因的调控网络,以及其在疾病发生发展中的作用,为相关疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Song, Yupu, Liu, Chang, Zhou, Yuxuan, Sui, Yujian, Sun, Yongfeng. 2022. Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose. In BMC genomics, 23, 821. doi:10.1186/s12864-022-09060-z. https://pubmed.ncbi.nlm.nih.gov/36510127/
2. Ma, Jun, Yang, Gan, Qin, Xuan, Li, Jian, Yin, Shi. 2024. Molecular characterization of MSX2 gene and its role in regulating steroidogenesis in yak (Bos grunniens) cumulus granulosa cells. In Theriogenology, 231, 101-110. doi:10.1016/j.theriogenology.2024.10.014. https://pubmed.ncbi.nlm.nih.gov/39427591/
3. Tan, D P, Nonaka, K, Nuckolls, G H, Slavkin, H C, Shum, L. . YY1 activates Msx2 gene independent of bone morphogenetic protein signaling. In Nucleic acids research, 30, 1213-23. doi:. https://pubmed.ncbi.nlm.nih.gov/11861914/
4. Plaisancié, Julie, Collet, Corinne, Pelletier, Valerie, Flori, Elisabeth, Dollfus, Hélène. 2014. MSX2 Gene Duplication in a Patient with Eye Development Defects. In Ophthalmic genetics, 36, 353-8. doi:10.3109/13816810.2014.886270. https://pubmed.ncbi.nlm.nih.gov/24666290/
5. Ruspita, Intan, Das, Pragnya, Miyoshi, Keiko, Snead, Malcolm L, Bei, Marianna. 2023. Enam expression is regulated by Msx2. In Developmental dynamics : an official publication of the American Association of Anatomists, 252, 1292-1302. doi:10.1002/dvdy.598. https://pubmed.ncbi.nlm.nih.gov/37191055/
6. Hornbachner, Ruth, Lackner, Andreas, Papuchova, Henrieta, Mechtler, Karl, Latos, Paulina A. . MSX2 safeguards syncytiotrophoblast fate of human trophoblast stem cells. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2105130118. https://pubmed.ncbi.nlm.nih.gov/34507999/
7. Babajko, Sylvie, de La Dure-Molla, Muriel, Jedeon, Katia, Berdal, Ariane. 2015. MSX2 in ameloblast cell fate and activity. In Frontiers in physiology, 5, 510. doi:10.3389/fphys.2014.00510. https://pubmed.ncbi.nlm.nih.gov/25601840/
8. Doi, Takashi, Puri, Prem, Bannigan, John, Thompson, Jennifer. . Msx1 and Msx2 gene expression is downregulated in the cadmium-induced omphalocele in the chick model. In Journal of pediatric surgery, 45, 1187-91. doi:10.1016/j.jpedsurg.2010.02.084. https://pubmed.ncbi.nlm.nih.gov/20620318/
9. Bidder, M, Latifi, T, Towler, D A. . Reciprocal temporospatial patterns of Msx2 and Osteocalcin gene expression during murine odontogenesis. In Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 13, 609-19. doi:. https://pubmed.ncbi.nlm.nih.gov/9556061/
10. Malewski, T, Milewicz, T, Krzysiek, J, Gregoraszczuk, E L, Augustowska, K. . Regulation of Msx2 gene expression by steroid hormones in human nonmalignant and malignant breast cancer explants cultured in vitro. In Cancer investigation, 23, 222-8. doi:. https://pubmed.ncbi.nlm.nih.gov/15945508/