Man2a1-flox 基因敲除小鼠

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产品名称

Man2a1-flox 基因敲除小鼠

产品编号

S-CKO-03643

品系全称

C57BL/6JCya-Man2a1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-17158-Man2a1-B6J-VA

品系状态

使用本品系发表的文献需注明: Man2a1-flox 基因敲除小鼠 mice (Strain S-CKO-03643) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
mannosidase 2, alpha 1
基因别称
Mana-2,Mana2,Map-2
染色体号
Chr 17 (Mouse)
转录本 ID
NCBI: NM_008549.2 | Ensembl: ENSMUST00000086723
修饰方式
条件性基因敲除
靶向范围
Exon 8
敲除长度
~678 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:104669Homozygous mutation of this gene results in premature death, dyserythropoiesis, systemic autoimmune disease, including an increase in serum immunoglobulins, glomerulonephritis, and hematuria,
Man2a1,也称为Mannosidase alpha class 2A member 1,是一种重要的α-甘露糖苷酶。α-甘露糖苷酶参与N-糖链的成熟过程,而N-糖链是细胞表面和分泌蛋白上的重要糖基化修饰。N-糖链在细胞信号传导、蛋白质折叠、免疫识别和细胞粘附等生物学过程中发挥重要作用。

Man2a1在多种肿瘤中表达异常,并与肿瘤的发生、发展和预后密切相关。研究表明,Man2a1在肝细胞癌、结直肠癌、卵巢癌、肺癌、食管癌、前列腺癌等多种肿瘤中表达上调。Man2a1的表达水平与肿瘤的淋巴结转移、远处转移和预后不良相关。Man2a1可能通过影响细胞增殖、凋亡、迁移和侵袭等生物学过程,促进肿瘤的发生和发展。

Man2a1还与免疫调节相关。研究发现,Man2a1的抑制可以增强肿瘤细胞对T细胞介导的杀伤作用的敏感性,并增强抗PD-L1治疗的疗效。Man2a1的抑制可以促进T细胞浸润到肿瘤组织中,从而提高抗肿瘤免疫反应。此外,Man2a1的抑制还可以与其他抗肿瘤药物协同作用,抑制肿瘤的生长和转移。

Man2a1还与某些疾病的发生和发展相关。例如,Man2a1的基因变异与精神分裂症的发生相关。Man2a1的抑制可以改善实验性结肠炎的症状,表明Man2a1可能参与肠道炎症的调节。

综上所述,Man2a1是一种重要的α-甘露糖苷酶,参与N-糖链的成熟过程,并与肿瘤的发生、发展和预后密切相关。Man2a1还与免疫调节和某些疾病的发生和发展相关。Man2a1的研究有助于深入理解N-糖链的生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略[1][2][3][4][5][6][7][8]。

参考文献:
1. Chen, Zhang-Hui, Yu, Yan P, Tao, Junyan, Michalopoulos, George K, Luo, Jian-Hua. 2017. MAN2A1-FER Fusion Gene Is Expressed by Human Liver and Other Tumor Types and Has Oncogenic Activity in Mice. In Gastroenterology, 153, 1120-1132.e15. doi:10.1053/j.gastro.2016.12.036. https://pubmed.ncbi.nlm.nih.gov/28245430/
2. Shi, Sailing, Gu, Shengqing, Han, Tong, Wucherpfennig, Kai W, Liu, X Shirley. 2020. Inhibition of MAN2A1 Enhances the Immune Response to Anti-PD-L1 in Human Tumors. In Clinical cancer research : an official journal of the American Association for Cancer Research, 26, 5990-6002. doi:10.1158/1078-0432.CCR-20-0778. https://pubmed.ncbi.nlm.nih.gov/32723834/
3. Wang, Yangzi, Zhao, Jianmin, Fu, Guoxiang, Yang, Fang, Jiang, Zhinong. . MAN2A1 predicts prognosis and progression through cancer-related pathways in colorectal cancer. In Translational cancer research, 11, 3686-3697. doi:10.21037/tcr-22-629. https://pubmed.ncbi.nlm.nih.gov/36388015/
4. Yu, Yan-Ping, Liu, Silvia, Nelson, Joel, Luo, Jian-Hua. 2021. Detection of fusion gene transcripts in the blood samples of prostate cancer patients. In Scientific reports, 11, 16995. doi:10.1038/s41598-021-96528-9. https://pubmed.ncbi.nlm.nih.gov/34417538/
5. Mealer, Robert G, Williams, Sarah E, Daly, Mark J, Cummings, Richard D, Smoller, Jordan W. 2020. Glycobiology and schizophrenia: a biological hypothesis emerging from genomic research. In Molecular psychiatry, 25, 3129-3139. doi:10.1038/s41380-020-0753-1. https://pubmed.ncbi.nlm.nih.gov/32377000/
6. Miao, Ran, Dong, Xingbei, Gong, Juanni, Zhong, Jiuchang, Yang, Yuanhua. 2020. Possible immune regulation mechanisms for the progression of chronic thromboembolic pulmonary hypertension. In Thrombosis research, 198, 122-131. doi:10.1016/j.thromres.2020.11.032. https://pubmed.ncbi.nlm.nih.gov/33316641/
7. Kader, Muhamuda, Yu, Yan-Ping, Liu, Silvia, Luo, Jian-Hua. 2024. Immuno-targeting the ectopic phosphorylation sites of PDGFRA generated by MAN2A1-FER fusion in HCC. In Hepatology communications, 8, . doi:10.1097/HC9.0000000000000511. https://pubmed.ncbi.nlm.nih.gov/39082961/
8. Yu, Yan-Ping, Liu, Silvia, Geller, David, Luo, Jian-Hua. 2024. Serum Fusion Transcripts to Assess the Risk of Hepatocellular Carcinoma and the Impact of Cancer Treatment through Machine Learning. In The American journal of pathology, 194, 1262-1271. doi:10.1016/j.ajpath.2024.02.017. https://pubmed.ncbi.nlm.nih.gov/38537933/