Slc6a4-flox 基因敲除小鼠

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产品名称

Slc6a4-flox 基因敲除小鼠

产品编号

S-CKO-03013

品系全称

C57BL/6JCya-Slc6a4em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-15567-Slc6a4-B6J-VA

品系状态

使用本品系发表的文献需注明: Slc6a4-flox 基因敲除小鼠 mice (Strain S-CKO-03013) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
基因别称
5-HTT,Htt,Sert
染色体号
Chr 11 (Mouse)
转录本 ID
NCBI: NM_010484 | Ensembl: ENSMUST00000021195
修饰方式
条件性基因敲除
靶向范围
Exon 3~4
敲除长度
~1.9 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:96285Homozygotes for a targeted null mutation exhibit greatly diminished brain serotonin levels and lack cortical barrel patterns. Also, mutants lack the locomotor enhancing response to the drug (+)-3,4-methylenedioxymethamphetamine.
SLC6A4,也称为5-HTT(5-羟色胺转运蛋白),是一种编码5-羟色胺转运蛋白的基因。5-羟色胺是一种重要的神经递质,参与调节情绪、认知、睡眠和食欲等多种生理和心理过程。SLC6A4基因位于人类17号染色体上,其启动子区域存在一个功能性多态性,即5-HTTLPR,由短等位基因(S)和长等位基因(L)组成。短等位基因的个体在遇到生活压力时,比长等位基因的个体更容易出现抑郁症状、可诊断的抑郁和自杀行为。这表明SLC6A4基因与抑郁症的发生密切相关,个体对环境压力的反应受其遗传组成的影响。SLC6A4基因还与情绪、内分泌和人格特征以及许多疾病有关,如自闭症、代谢综合征和哮喘。SLC6A4基因在基因×基因(G×G)和基因×环境(G×E)相互作用中发挥重要作用,这些相互作用对健康和疾病的影响尚不完全清楚。SLC6A4基因的表达和功能受到表观遗传调控,如DNA甲基化,这可能与抑郁症的发生有关。此外,SLC6A4基因的变异还可能影响其他基因的表达,如MAOA和CLOCK基因,从而影响个体的生理和行为特征。SLC6A4基因的研究有助于深入理解5-羟色胺系统的生物学功能和疾病发生机制,为相关疾病的治疗和预防提供新的思路和策略[1][2][3][4][5][6][7][8][9][10]。

参考文献:
1. Caspi, Avshalom, Sugden, Karen, Moffitt, Terrie E, Braithwaite, Antony, Poulton, Richie. . Influence of life stress on depression: moderation by a polymorphism in the 5-HTT gene. In Science (New York, N.Y.), 301, 386-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12869766/
2. Murphy, Dennis L, Moya, Pablo R. 2011. Human serotonin transporter gene (SLC6A4) variants: their contributions to understanding pharmacogenomic and other functional G×G and G×E differences in health and disease. In Current opinion in pharmacology, 11, 3-10. doi:10.1016/j.coph.2011.02.008. https://pubmed.ncbi.nlm.nih.gov/21439906/
3. Ma, D Q, Rabionet, R, Konidari, I, Pericak-Vance, M A, Martin, E R. . Association and gene-gene interaction of SLC6A4 and ITGB3 in autism. In American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 153B, 477-483. doi:10.1002/ajmg.b.31003. https://pubmed.ncbi.nlm.nih.gov/19588468/
4. Sookoian, Silvia, Gianotti, Tomas Fernandez, Burgueño, Adriana, Pirola, Carlos Jose. . Gene-gene interaction between serotonin transporter (SLC6A4) and CLOCK modulates the risk of metabolic syndrome in rotating shiftworkers. In Chronobiology international, 27, 1202-18. doi:10.3109/07420528.2010.496913. https://pubmed.ncbi.nlm.nih.gov/20653450/
5. Mendonça, M S, Mangiavacchi, P M, De Sousa, P F, Kanashiro, M M, Rios, A F L. 2018. Epigenetic variation at the SLC6A4 gene promoter in mother-child pairs with major depressive disorder. In Journal of affective disorders, 245, 716-723. doi:10.1016/j.jad.2018.10.369. https://pubmed.ncbi.nlm.nih.gov/30447571/
6. Guo, Yanli, Zhao, Jing, Xu, Qinglei, Schinckel, Allan P, Zhou, Bo. . Identification of functional single nucleotide polymorphisms in the porcine SLC6A4 gene associated with aggressive behavior in weaned pigs after mixing. In Journal of animal science, 100, . doi:10.1093/jas/skac131. https://pubmed.ncbi.nlm.nih.gov/35419600/
7. Kravić, Nermina, Šabić Džananović, Emina, Muminović Umihanić, Mirnesa, Deckert, Jürgen, Avdibegović, Esmina. . Association Analysis of Maoa and Slc6a4 Gene Variation in South East European War Related Posttraumatic Stress Disorder. In Psychiatria Danubina, 31, 211-218. doi:10.24869/psyd.2019.211. https://pubmed.ncbi.nlm.nih.gov/31291228/
8. Chen, Gao, Xu, Jianguo, Wei, Shuai, Zhao, Chunru, Ma, Yingjiao. . [Association of SLC6A4 gene c.*670T>G polymorphism with the risk for asthma and peripheral blood cytological characteristics among ethnic Zhuang Chinese population]. In Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 40, 1228-1235. doi:10.3760/cma.j.cn511374-20221001-00666. https://pubmed.ncbi.nlm.nih.gov/37730222/
9. Lam, Dilys, Ancelin, Marie-Laure, Ritchie, Karen, Saffery, Richard, Ryan, Joanne. 2018. Genotype-dependent associations between serotonin transporter gene (SLC6A4) DNA methylation and late-life depression. In BMC psychiatry, 18, 282. doi:10.1186/s12888-018-1850-4. https://pubmed.ncbi.nlm.nih.gov/30180828/
10. Hernández-Muñoz, S, Camarena-Medellin, B, González-Macías, L, Flores-Ramos, M, Caballero-Romo, A. 2020. Sequence analysis of five exons of SLC6A4 gene in Mexican patients with anorexia nervosa and bulimia nervosa. In Gene, 748, 144675. doi:10.1016/j.gene.2020.144675. https://pubmed.ncbi.nlm.nih.gov/32315729/