Fbln1-flox 基因敲除小鼠

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产品名称

Fbln1-flox 基因敲除小鼠

产品编号

S-CKO-02367

品系全称

C57BL/6JCya-Fbln1em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-14114-Fbln1-B6J-VA

品系状态

使用本品系发表的文献需注明: Fbln1-flox 基因敲除小鼠 mice (Strain S-CKO-02367) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
fibulin 1
基因别称
-
染色体号
Chr 15 (Mouse)
转录本 ID
NCBI: NM_010180 | Ensembl: ENSMUST00000057410
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~1.5 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:95487Mice homozygous for disruption of this gene develop problems with spontaneous bleeding as embryos. Most die within the first two days of life. Those that survive this period develop normally and eventually recover from their early developmental abnormalities.
Fbln1,也称为纤维蛋白-1,是一种钙结合的糖蛋白,存在于细胞外基质(ECM)和血清中。Fbln1基因位于人类染色体22q13.2-q13.3和鼠类染色体15的E-F带[5]。Fbln1蛋白在ECM中发挥重要作用,参与细胞迁移、粘附、增殖和分化等多种生物学过程。

Fbln1在多种疾病中发挥重要作用,包括黑色素瘤、舌鳞状细胞癌、乳腺癌和阿尔茨海默病。研究表明,Fbln1在黑色素瘤中表达下调,与患者预后不良相关[1]。Fbln1启动子区域的高甲基化是Fbln1表达下调的主要原因之一[2]。Fbln1在舌鳞状细胞癌中同样表达下调,其启动子区域的高甲基化与肿瘤进展和患者预后不良相关[6]。Fbln1在乳腺癌中表达下调,其表达水平与患者预后相关,Fbln1可能作为乳腺癌的预后指标和潜在的治疗靶点[8]。在阿尔茨海默病中,Fbln1的表达上调,其异常甲基化状态与疾病发生发展相关[7]。

Fbln1在正常和异常的胎盘发育中也发挥重要作用。研究表明,Fbln1在正常胎盘发育过程中表达,并在异常胎盘发育过程中表达上调[9]。Fbln1在胚胎发育过程中参与细胞外基质的构建,对胚胎器官的形成和发育至关重要。

Fbln1在骨再生中也发挥重要作用。研究表明,Fbln1促进脐带间充质干细胞的成骨分化,并促进骨再生[3]。Fbln1通过m6A修饰和miRNA调控机制影响干细胞的成骨分化潜能[3]。

Fbln1在软骨细胞增殖中也发挥重要作用。研究表明,Fbln1通过增加Smad2的磷酸化水平促进软骨细胞增殖[4]。Fbln1在骨关节炎患者中表达上调,可能参与骨关节炎的发生和发展[4]。

综上所述,Fbln1是一种重要的ECM蛋白,参与多种生物学过程和疾病的发生发展。Fbln1在黑色素瘤、舌鳞状细胞癌、乳腺癌和阿尔茨海默病中表达异常,与患者预后不良相关。Fbln1在胎盘发育、骨再生和软骨细胞增殖中也发挥重要作用。深入研究Fbln1的生物学功能和调控机制,有助于揭示其与疾病发生发展的关系,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Liu, Xiao-Tian, Liu, Tian-Tian, Wu, Meng-Yuan, Zhuang, Jiang-Xing, Wang, Qin. . Identifying FBLN1 (Gene ID: 2192) as a Potential Melanoma Biomarker for Melanoma based on an Analysis of microRNA Expression Profiles in the GEO and TCGA Databases. In Genetic testing and molecular biomarkers, 25, 68-78. doi:10.1089/gtmb.2020.0274. https://pubmed.ncbi.nlm.nih.gov/33470885/
2. Wu, Bao-Jin, Zhou, Zhao-Ping, Li, Wen-Peng, Liu, Qing-Feng, Jiang, Hua. 2013. Abnormal hypermethylation and clinicopathological significance of FBLN1 gene in cutaneous melanoma. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35, 123-7. doi:10.1007/s13277-013-1015-5. https://pubmed.ncbi.nlm.nih.gov/23907575/
3. Yang, Haoqing, Wang, Wanqing, Liu, Huina, Jin, Luyuan, Fan, Zhipeng. 2024. miR615-3p inhibited FBLN1 and osteogenic differentiation of umbilical cord mesenchymal stem cells by associated with YTHDF2 in a m6A-miRNA interaction manner. In Cell proliferation, 57, e13607. doi:10.1111/cpr.13607. https://pubmed.ncbi.nlm.nih.gov/38353178/
4. Xu, Gang, Geng, Xiang, Yang, Fan, Zhang, Haijiao. 2021. FBLN1 promotes chondrocyte proliferation by increasing phosphorylation of Smad2. In Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 27, 242-248. doi:10.1016/j.jos.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33610427/
5. Mattei, M G, Pan, T C, Zhang, R Z, Timpl, R, Chu, M L. . The fibulin-1 gene (FBLN1) is located on human chromosome 22 and on mouse chromosome 15. In Genomics, 22, 437-8. doi:. https://pubmed.ncbi.nlm.nih.gov/7806231/
6. Yang, Fang, Shi, Jing, Zhang, Liangyu, Wang, Hongyan, Li, Yang. 2021. Role of fibulin-1 gene promoter methylation in the carcinogenesis and development of tongue squamous cell carcinoma. In Oral surgery, oral medicine, oral pathology and oral radiology, 133, 432-440. doi:10.1016/j.oooo.2021.10.006. https://pubmed.ncbi.nlm.nih.gov/35153187/
7. Tang, Rui, Jian, Yue, Hu, Zhimin, Yang, Zhihui, Tang, Mingxi. 2024. A Bioinformatics-Based Study on Methylation Alterations of the FBLN1 Gene in Hippocampal Tissue of Alzheimer's Disease Model DKO and DTG Mice. In International journal of molecular sciences, 25, . doi:10.3390/ijms25169036. https://pubmed.ncbi.nlm.nih.gov/39201719/
8. Tian, Wenwen, Luo, Yongzhou, Tang, Yuhui, Xie, Xiaoming, Wei, Weidong. 2023. Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration. In International journal of biological sciences, 19, 1645-1663. doi:10.7150/ijbs.81939. https://pubmed.ncbi.nlm.nih.gov/37056938/
9. Singh, U, Sun, T, Larsson, T, Kostka, G, Fundele, R H. 2005. Expression and functional analysis of fibulin-1 (Fbln1) during normal and abnormal placental development of the mouse. In Placenta, 27, 1014-21. doi:. https://pubmed.ncbi.nlm.nih.gov/16338003/