Crlf1-flox 基因敲除小鼠

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产品名称

Crlf1-flox 基因敲除小鼠

产品编号

S-CKO-01868

品系全称

C57BL/6NCya-Crlf1em1flox/Cya

品系背景

C57BL/6NCya

品系编号

CKOCMP-12931-Crlf1-B6N-VA

品系状态

使用本品系发表的文献需注明: Crlf1-flox 基因敲除小鼠 mice (Strain S-CKO-01868) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
cytokine receptor-like factor 1
基因别称
CLF-1,CRLM-3,CRLM3,NR6,NR6.1
染色体号
Chr 8 (Mouse)
转录本 ID
NCBI: NM_018827 | Ensembl: ENSMUST00000008032
修饰方式
条件性基因敲除
靶向范围
Exon 2~6
敲除长度
~3.4 kb
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:1340030Mice homozygous for a targeted mutation fail to suckle effectively and do not survive beyond 24 hrs after birth. Newborns exhibit reduced numbers of hematopoietic progenitor cells as well as a significant reduction in the number of motoneurons in the lumbar spinal cord and facial nucleus.
CRLF1,即细胞因子受体样因子1,是一种可溶性I型细胞因子受体,它在多种生物学过程中发挥着关键作用。CRLF1与细胞因子CLCF1(cardiotrophin-like cytokine factor 1)的相互作用能够触发细胞内的信号传导级联反应,进而影响基因表达,导致细胞增殖、分化和活化的变化。CRLF1在生理和病理条件下均具有重要作用,尤其是在Crisponi/cold-induced sweating syndrome(CS/CISS)等疾病中[1]。

在甲状腺癌的研究中,发现CRLF1能够通过与MYH9的相互作用,通过ERK/ETV4轴促进甲状腺癌细胞的增殖和转移[2]。此外,CRLF1的表达抑制能够促进骨髓间充质干细胞的软骨生成分化,并通过激活miR-320来保护软骨组织免受骨关节炎的损伤[4]。在骨关节炎的研究中,还发现miR-8485能够抑制CRLF1的表达,从而抑制IL-1β触发的软骨细胞炎症[3]。

在皮肤疾病的研究中,CRLF1和NRG1被鉴定为与肥厚性瘢痕形成相关的免疫相关特征基因[5]。在CRLF1突变导致CS/CISS1综合征的研究中,发现了新的CRLF1突变,并创建了CRLF1突变数据库,进一步扩展了CRLF1突变的范围[6]。此外,还报道了印度新生儿CRLF1基因突变导致的Crisponi综合征的病例[7]。

在骨软骨修复的研究中,发现CRLF1能够促进骨髓间充质干细胞中软骨生成分化,并通过抑制软骨细胞中的分解代谢事件来增强骨软骨缺损的修复[8]。在动脉粥样硬化的研究中,发现吸烟与女性和男性动脉粥样硬化斑块中CRLF1表达的上调有关,这可能是女性吸烟者心血管风险升高的原因[9]。

综上所述,CRLF1在多种生物学过程中发挥着重要作用,包括细胞增殖、分化和活化,以及免疫反应等。CRLF1的表达和功能失调与多种疾病的发生和发展密切相关,包括甲状腺癌、骨关节炎、肥厚性瘢痕和动脉粥样硬化等。CRLF1的研究有助于深入理解其生物学功能和疾病发生机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Crisponi, Laura, Buers, Insa, Rutsch, Frank. 2022. CRLF1 and CLCF1 in Development, Health and Disease. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020992. https://pubmed.ncbi.nlm.nih.gov/35055176/
2. Yu, Shi-Tong, Sun, Bai-Hui, Ge, Jun-Na, Chen, Wei-Sheng, Lei, Shang-Tong. 2020. CRLF1-MYH9 Interaction Regulates Proliferation and Metastasis of Papillary Thyroid Carcinoma Through the ERK/ETV4 Axis. In Frontiers in endocrinology, 11, 535. doi:10.3389/fendo.2020.00535. https://pubmed.ncbi.nlm.nih.gov/32982961/
3. Yang, Guang, Ji, Bingzhou, Li, Hengzhen, Liu, Shuguang, Xiao, Wenfeng. 2024. Inhibition of CRLF1 expression by miR-8485 alleviates IL-1β-induced chondrocyte inflammation, apoptosis, and extracellular matrix degradation. In International immunopharmacology, 144, 113643. doi:10.1016/j.intimp.2024.113643. https://pubmed.ncbi.nlm.nih.gov/39580860/
4. Xu, Hao, Ding, Changrong, Guo, Cuicui, Luo, Bing, Xiang, Hongfei. 2021. Suppression of CRLF1 promotes the chondrogenic differentiation of bone marrow-derived mesenchymal stem and protects cartilage tissue from damage in osteoarthritis via activation of miR-320. In Molecular medicine (Cambridge, Mass.), 27, 116. doi:10.1186/s10020-021-00369-1. https://pubmed.ncbi.nlm.nih.gov/34551709/
5. Yu, Boya, Cao, Yalei, Li, Shiyi, Zhang, Lixia, Chen, Minliang. 2024. Identification and validation of CRLF1 and NRG1 as immune-related signatures in hypertrophic scar. In Genomics, 116, 110797. doi:10.1016/j.ygeno.2024.110797. https://pubmed.ncbi.nlm.nih.gov/38262564/
6. Piras, Roberta, Chiappe, Francesca, Torraca, Ilaria La, Crisponi, Laura, Rutsch, Frank. 2014. Expanding the mutational spectrum of CRLF1 in Crisponi/CISS1 syndrome. In Human mutation, 35, 424-33. doi:10.1002/humu.22522. https://pubmed.ncbi.nlm.nih.gov/24488861/
7. A, Rekha, Chandran, Shanu, Ghatak, Arpita Roy, Thomas, Niranjan, Danda, Sumita. 2022. Novel Mutations in CRLF1: Case Reports with Crisponi Syndrome. In Indian journal of pediatrics, 89, 1148-1150. doi:10.1007/s12098-022-04263-4. https://pubmed.ncbi.nlm.nih.gov/35984556/
8. Zhang, Fenglin, Clair, Andrew J, Dankert, John F, Campbell, Kirk A, Kirsch, Thorsten. 2024. Cytokine Receptor-like Factor 1 (CRLF1) and Its Role in Osteochondral Repair. In Cells, 13, . doi:10.3390/cells13090757. https://pubmed.ncbi.nlm.nih.gov/38727293/
9. Lan, Tian, Palm, Kaylin C A, Hoeben, Luka, Pasterkamp, Gerard, Mokry, Michal. 2024. Tobacco smoking is associated with sex- and plaque-type specific upregulation of CRLF1 in atherosclerotic lesions. In Atherosclerosis, 397, 118554. doi:10.1016/j.atherosclerosis.2024.118554. https://pubmed.ncbi.nlm.nih.gov/39137621/