Cpa3-flox 基因敲除小鼠

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产品名称

Cpa3-flox 基因敲除小鼠

产品编号

S-CKO-01841

品系全称

C57BL/6JCya-Cpa3em1flox/Cya

品系背景

C57BL/6JCya

品系编号

CKOCMP-12873-Cpa3-B6J-VA

品系状态

使用本品系发表的文献需注明: Cpa3-flox 基因敲除小鼠 mice (Strain S-CKO-01841) were purchased from Cyagen.
交付类型
周龄
性别
基因型
数量
cKO小鼠库模型

基本信息

基因研究概述

质控标准

基因
基因全称
carboxypeptidase A3, mast cell
基因别称
MC-CPA
染色体号
Chr 3 (Mouse)
转录本 ID
NCBI: NM_007753.2 | Ensembl: ENSMUST00000001921
修饰方式
条件性基因敲除
靶向范围
Exon 3
敲除长度
~625 bp
品系说明
该品系是基于策略设计时的数据库信息制作而成,建议您在购买前查询最新的数据库和相关文献,以获取最准确的表型信息。
表型提示
MGI:88479Homozygous null mice have immature peritoneal mast cells but normal mast cell functions.
Cpa3,即羧肽酶A3,是一种在哺乳动物免疫系统中具有重要作用的金属羧肽酶。该基因编码的蛋白质在肥大细胞中高度表达,并储存在肥大细胞的丰富细胞质颗粒中。Cpa3属于M14A亚家族的金属羧肽酶,与其他消化酶如CPA1、CPA2、CPB1和CPO具有结构相似性。Cpa3在组织稳态、重塑和炎症中发挥重要作用,其表达和功能与多种疾病相关。

Cpa3在多种癌症中表达异常,包括肺腺癌、前列腺癌和结直肠癌。在肺腺癌中,Cpa3的表达与患者的预后和免疫治疗效果相关。一项研究表明,Cpa3是肺腺癌风险模型中的关键预后基因之一,其表达水平与患者的生存期和免疫浸润程度相关[1]。在前列腺癌中,Cpa3的表达受组蛋白去乙酰化酶抑制剂调控,参与前列腺癌细胞的分化过程[2]。在结直肠癌中,Cpa3的表达与肥大细胞的活化和肿瘤微环境的调节相关,并与患者的预后相关[3]。

Cpa3在肺部疾病中也发挥重要作用。在慢性阻塞性肺疾病(COPD)和特发性肺纤维化(IPF)中,Cpa3的表达水平显著升高,并与免疫病理学改变和肺功能下降相关[4]。此外,Cpa3在哮喘和COPD的炎症亚型中也具有重要作用。研究发现,Cpa3的表达与哮喘患者对口服皮质类固醇的响应相关,并在COPD中与嗜酸性粒细胞炎症和肺功能下降相关[5,6,7,8,9,10]。

综上所述,Cpa3是一种重要的金属羧肽酶,在免疫系统和肺部疾病中发挥重要作用。Cpa3的表达和功能与多种疾病相关,包括癌症、COPD和哮喘。Cpa3的研究有助于深入理解免疫系统和肺部疾病的发病机制,为疾病的治疗和预防提供新的思路和策略。

参考文献:
1. Shu, Jianfeng, Jiang, Jinni, Zhao, Guofang. 2023. Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis. In Frontiers in immunology, 14, 1177847. doi:10.3389/fimmu.2023.1177847. https://pubmed.ncbi.nlm.nih.gov/37583701/
2. Huang, H, Reed, C P, Zhang, J S, Wang, L, Smith, D I. . Carboxypeptidase A3 (CPA3): a novel gene highly induced by histone deacetylase inhibitors during differentiation of prostate epithelial cancer cells. In Cancer research, 59, 2981-8. doi:. https://pubmed.ncbi.nlm.nih.gov/10383164/
3. Xie, Zhenyu, Niu, Liaoran, Zheng, Gaozan, Zhang, Jian, Zheng, Jianyong. 2023. Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment. In Cell & bioscience, 13, 217. doi:10.1186/s13578-023-01144-x. https://pubmed.ncbi.nlm.nih.gov/38031173/
4. Siddhuraj, Premkumar, Jönsson, Jimmie, Alyamani, Manar, Lindstedt, Sandra, Erjefält, Jonas S. 2022. Dynamically upregulated mast cell CPA3 patterns in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. In Frontiers in immunology, 13, 924244. doi:10.3389/fimmu.2022.924244. https://pubmed.ncbi.nlm.nih.gov/35983043/
5. Southworth, Thomas, Jevnikar, Zala, McCrae, Christopher, Singh, Dave. 2022. A sputum 6-gene signature predicts airway inflammation endotypes and exacerbation frequency in chronic obstructive pulmonary disease. In Biomarkers in medicine, 16, 277-289. doi:10.2217/bmm-2021-0653. https://pubmed.ncbi.nlm.nih.gov/35176870/
6. Akula, Srinivas, Hellman, Lars, Avilés, Francesc Xavier, Wernersson, Sara. 2021. Analysis of the mast cell expressed carboxypeptidase A3 and its structural and evolutionary relationship to other vertebrate carboxypeptidases. In Developmental and comparative immunology, 127, 104273. doi:10.1016/j.dci.2021.104273. https://pubmed.ncbi.nlm.nih.gov/34619175/
7. Marchi, Emanuele, Hinks, Timothy S C, Richardson, Matthew, Choy, David F, Bradding, Peter. 2024. The effects of inhaled corticosteroids on healthy airways. In Allergy, 79, 1831-1843. doi:10.1111/all.16146. https://pubmed.ncbi.nlm.nih.gov/38686450/
8. Winter, Natasha A, Gibson, Peter G, McDonald, Vanessa M, Fricker, Michael. 2021. Sputum Gene Expression Reveals Dysregulation of Mast Cells and Basophils in Eosinophilic COPD. In International journal of chronic obstructive pulmonary disease, 16, 2165-2179. doi:10.2147/COPD.S305380. https://pubmed.ncbi.nlm.nih.gov/34321876/
9. Berthon, Bronwyn S, Gibson, Peter G, Wood, Lisa G, MacDonald-Wicks, Lesley K, Baines, Katherine J. 2017. A sputum gene expression signature predicts oral corticosteroid response in asthma. In The European respiratory journal, 49, . doi:10.1183/13993003.00180-2017. https://pubmed.ncbi.nlm.nih.gov/28663317/
10. Frøssing, Laurits, Silberbrandt, Alexander, Von Bülow, Anna, Baines, Katherine J, Porsbjerg, Celeste. 2021. Airway gene expression identifies subtypes of type 2 inflammation in severe asthma. In Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 52, 59-69. doi:10.1111/cea.13966. https://pubmed.ncbi.nlm.nih.gov/34142396/